Article (Scientific journals)
G-quadruplexes in telomeric repeats are conserved in a solvent-free environment
Baker, Erin Shammel; Bernstein, Summer L; Gabelica, Valérie et al.
2006In International Journal of Mass Spectrometry, 253 (3), p. 225-237
Peer Reviewed verified by ORBi
 

Files


Full Text
2006-IJMS-253-p225_G4collBowers_postprint.pdf
Author postprint (696.27 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
ion mobility; G-quadruplexes; DNA; mass spectrometry
Abstract :
[en] The structural properties of G-quadruplex forming sequences. such as the human telomeric repeat d(T(2)AG(3))(n), are of great interest due to their role in cancer and cellular aging. To determine if G-quadruplexes are present in a solvent-free environment. different lengths of the telomeric repeat d(T(2)AG(3))(n) (where n = 1, 2, 4 and 6) and dTG(4)T were investigated with mass specteometry, ion mobility and molecular dynamics calculations. Nano-ESI-MS illustrated quadruples stoichiometries compatible with G-quadruplex Structure.; for each sequence, with dT(2)AG(3) and dTG(4)T forming 4-strand complexes with two and three NH4+ adducts. d(T(2)AG(3))(2) a 2-strand complex. and d(T(2)AG(3))(4) and d(T(2)AG(3))(6) remaining single-stranded. Experimental cross sections were obtained for all species using ion mobility method.,. In all case.,. these could be quantitatively matched to model cross sections with specific strand orientations (parallel/antiparallel) and structures. For each species, the solvent-free structures agreed with the solution CD measurements, but the ion mobility/modeling procedure often gave Much more detailed structural information. (C) 2006 Elsevier B.V. All rights reserved.
Research center :
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Disciplines :
Chemistry
Author, co-author :
Baker, Erin Shammel;  University of California Santa Barbara - UCSB > Department of Chemistry and Biochemistry
Bernstein, Summer L;  University of California Santa Barbara - UCSB > Department of Chemistry and Biochemistry
Gabelica, Valérie ;  Université de Liège - ULiège > Département de Chimie (Sciences) > Chimie physique, spectrométrie de masse
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de Chimie (Sciences) > Chimie physique, spectrométrie de masse
Bowers, Michael T;  University of California Santa Barbara - UCSB > Department of Chemistry and Biochemistry
Language :
English
Title :
G-quadruplexes in telomeric repeats are conserved in a solvent-free environment
Publication date :
2006
Journal title :
International Journal of Mass Spectrometry
ISSN :
1387-3806
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
253
Issue :
3
Pages :
225-237
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Commentary :
This is a postprint author version of an article published as: E.S. Baker, S.L. Bernstein, V. Gabelica, E. De Pauw, M.T. Bowers, " G-Quadruplexes in Telomeric Repeats Are Conserved in a Solvent-Free Environment", Int. J. Mass Spectrom. (2006), 253(3) Special Issue "Solution vs. Gas Phase Biomolecule Structure", 225-237.
Available on ORBi :
since 16 September 2008

Statistics


Number of views
194 (5 by ULiège)
Number of downloads
236 (2 by ULiège)

Scopus citations®
 
81
Scopus citations®
without self-citations
52
OpenCitations
 
76

Bibliography


Similar publications



Contact ORBi