Article (Scientific journals)
Fib3-3 as a Biomarker for Osteoarthritis in a Rat Model with Metabolic Dysregulation.
de Visser, Huub M.; Sanchez, Christelle; Mastbergen, Simon C. et al.
2019In Cartilage, 10 (3), p. 329-334
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Keywords :
animal model; articular cartilage; osteoarthritis; biomarker
Abstract :
[en] Objective Fibulin-3 is a glycoprotein highly expressed in osteoarthritic cartilage and inhibits angiogenesis and chondrocyte differentiation. Recent studies have indicated that fibulin-3 has potential value as a biomarker in osteoarthritis. The aim of the present study is to examine the role of 3 fibulin-3 peptides (Fib3-1, Fib3-2, and Fib3-3) and a type II collagen degradation product in a rat osteoarthritis model with systemic metabolic alterations combined with local cartilage damage. Design Forty, 12-week-old male, Wistar rats were randomly divided over 2 groups: a standard or a high-fat diet inducing metabolic dysregulation. After 12 weeks, articular cartilage damage was induced on the femoral condyles (groove model), in 1 knee joint in 14 rats of each diet group. At endpoint, blood was collected and serum was isolated. Enzyme-linked immunosorbent assay on all selected fibulin-3 fragments was performed from serum samples in addition to immunohistochemical analysis for Fib3-3. Results Serum concentrations of Fib3-3 were increased by 29.9%, when cartilage damage was induced in addition to a high-fat diet. Fib3-3 was also associated with an increased histological total joint degeneration ( r = 0.435) and cartilage degeneration ( r = 0.435). Immunostainings demonstrated increased Fib3-3 in the superficial cartilage of animals with high-fat diet and/or cartilage damage. Conclusions In the rat groove model combined with high-fat diet-induced metabolic dysregulation an increased Fib3-3 concentration was observed systemically, which is associated with local joint degeneration. This suggests that systemic Fib3-3 concentrations can indicate the status of joint degeneration and function as a biomarker in osteoarthritis.
Research center :
d‐BRU - Dental Biomaterials Research Unit - ULiège [BE]
Disciplines :
Rheumatology
Author, co-author :
de Visser, Huub M.
Sanchez, Christelle  ;  Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartilage (U.R.O.C.)
Mastbergen, Simon C.
Lafeber, Floris P. J. G.
Henrotin, Yves  ;  Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartilage (U.R.O.C.)
Weinans, Harrie
Language :
English
Title :
Fib3-3 as a Biomarker for Osteoarthritis in a Rat Model with Metabolic Dysregulation.
Publication date :
2019
Journal title :
Cartilage
ISSN :
1947-6035
eISSN :
1947-6043
Publisher :
SAGE Publications, Thousand Oaks, United States - California
Volume :
10
Issue :
3
Pages :
329-334
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
FP7 - 305815 - D-BOARD - Novel Diagnostics and Biomarkers for Early Identification of Chronic Inflammatory Joint Diseases
Funders :
CE - Commission Européenne [BE]
Available on ORBi :
since 19 December 2017

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