[en] Methane (CH4) naturally produced by dairy cows during ruminal fermentation is an important greenhouse gas. An equation based on 446 reference data has been developed to predict easily individual CH4 emissions from milk mid-infrared (MIR) spectra. This equation was based on CH4 data measured exclusively with the SF6 technique on 146 distinct Holstein, Jersey and Holstein×Jersey cows. As breeds, managements, diets, etc. are different from one geographical area to another, representative reference data have to be included in the calibration set before applying this equation in a location. However, the local CH4 data needed are likely to be collected with different techniques (chambers, GreenFeed, etc.) depending on the research team and its equipment. A first study has therefore been conducted (1) to test the performance of the actual equation on data obtained in open-circuit chambers and (2) to analyse the impact of the inclusion of these data in the calibration set. A total of 60 chamber measurements of CH4 and milk MIR spectra were obtained from 30 lactating Brown-Swiss cows. The correlation between actually measured and predicted CH4 (C1) was 0.48. This result is in the range of expectations given the R2c of the equation (0.75), the correlation known between SF6 and chamber methods (~0.80), and the breed and diet differing between calibration sets. The correlation was about 0.70 after the inclusion of the chamber data (and so the inherent variability) in the calibration set (C2). As chambers are known as the gold standard method, the C1 observed confirms the relevance of using milk MIR technique. Moreover, C2 is very encouraging regarding the possibility to include data coming from chambers into the existing CH4 equation.