[en] Plasma Membrane is the primary structure for adjusting to ever changing conditions. PM sub-compartmentalization in domains is thought to orchestrate signalling cascades. Yet, mechanisms governing membrane organization are mostly uncharacterized. The plant-specific proteins REMORINs are factors regulating hormonal crosstalk and host invasion. REMs are the best-characterized PM nanodomain markers targeted to the PM via an uncharacterized moiety called REMORIN C-terminal Anchor. By coupling biophysical methods, super-resolution microscopy and physiology, we decipher an original mechanism regulating the dynamic and organization of nanodomains. We showed that PM targeting is independent of the COP II-dependent secretory pathway and mediated by PI4P and sterol. REM-CA is an unconventional lipid-binding motif that confers nanodomain organization. Analyses of REM-CA mutants by single particle tracking demonstrate that mobility and supramolecular organization are critical for immunity. This study provides a unique mechanistic insight into how the tight control of spatial segregation is critical in the definition of PM domain necessary to support biological function.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Gronnier, Julien ✱
Crowet, Jean-Marc ✱; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Habenstein, Birgit ✱
Nasir, Mehmet Nail ✱; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie générale et organique
Bayle, Vincent
Hosy, Eric
Platre, Matthieu Pierre
Gouguet, Paul
Raffaele, Sylvain
Martinez, Denis
Grelard, Axelle
Loquet, Antoine
Simon-Plas, Françoise
Gerbeau-Pissot, Patricia
Der, Christophe
Bayer, Emmanuelle
Jaillais, Yvon
Deleu, Magali ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Germain, Veronique; Université Bordeaux 1 > LBM
Lins, Laurence ✱; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Mongrand, Sebastien ✱; Université de Bordeaux > LBM
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