Allelic variation in TLR4 is linked to resistance to Salmonella Enteritidis infection in chickens.

[en] Salmonella Enteritidis (SE) is a foodborne pathogen that negatively affects both animal and human health. Polymorphisms of the TLR4 gene may affect recognition by Toll-like receptor 4 (TLR4) of bacterial lipopolysaccharide (LPS), leading to differences in host resistance to pathogenic infections. The present study has investigated polymorphic loci of chicken TLR4 (ChTLR4) in ten chicken breeds, electrostatic potentials of mutant structures of TLR4, and a linkage analysis between allelic variation and survival ratio to infection with SE in specific-pathogen-free (SPF) White Leghorns. A total of 19 Single Nucleotide Polymorphisms (SNPs), of which 10 were novel, were found in chicken breeds. Seven newly identified amino acid variants (C68G, G674A, G782A, A896T, T959G, T986A, and A1104C) and previously reported important mutations (G247A, G1028A, C1147T, and A1832G) were demonstrated in the extracellular domain of the ChTLR4 gene. Significant changes in surface electrostatic potential of the ectodomain of TLR4, built by homology modeling, were observed at the Glu83Lys (G247A), Arg298Ser (A896T), Ser368Arg (A1104C), and Gln611Arg (A1832G) substitutions. Linkage analysis showed that one polymorphic locus G247A of TLR4 gene, common in all breeds examined, was significantly associated with increased resistance to SE in SPF White Leghorns chicks (log-rank P-value = 0.04). The genotypes from A1832G SNPs did not show statistically significant survival differences. This study has provided the first direct evidence that G247A substitution in ChTLR4 is associated with increased resistance to Salmonella Enteritidis.

Disciplines :

Animal production & animal husbandry

Author, co-author :

Li, Peng ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)

Wang, Huihua

Zhao, Xingwang

Gou, Zhongyong

Liu, Ranran

Song, Yongmei

Li, Qinghe

Zheng, Maiqing

Cui, Huanxian

Everaert, Nadia ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions animales et nutrition

Zhao, Guiping

Wen, Jie

Language :

English

Title :

Allelic variation in TLR4 is linked to resistance to Salmonella Enteritidis infection in chickens.

Publication date :

2017

Journal title :

Poultry Science

ISSN :

0032-5791

eISSN :

1525-3171

Publisher :

Poultry Science Association, United States - Illinois

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 05 July 2017

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