Poster (Scientific congresses and symposiums)
Identifying infectious agents from lung transplant patients using GC×GC-TOFMS
Stefanuto, Pierre-Hugues; Rees, Christiaan A.; Romamno, Rosalba et al.
2017International Symposium on Capillary Chromatography and GCxGC symposium
 

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Keywords :
GCxGC; Volatile organic compounds; Transplant
Abstract :
[en] During the first year post-surgery, bacterial and fungal infections are the main causes of death in lung transplant recipients. Contributing substantially to the high mortality rate (35%) is the lengthy time-to-diagnosis, which includes both pathogen identification and antibiotic resistance profiling. A novel diagnostic strategy based on the detection of volatile compounds in breath seems a promising option to improve these patients’ outcomes. In order to demonstrate the feasibility of this approach, we characterized the volatile fraction of different clinical matrices (i.e., bronchoalveolar lavage (BAL) and blind bronchial aspirate (BBA)). Due to the complexity of the mixture and high dynamic range of the concentrations, we applied headspace solid phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC-TOFMS) to obtain a comprehensive overview of the sample headspace composition. Following the analytical step, we applied various univariate and multivariate statistical methods (e.g. random forest, principal component analysis, and hierarchical cluster analysis) in order to extract required information from the data. Based on the composition of the volatile fraction, we were able to differentiate between BAL and BBA with a classification accuracy of 88.4 %. Furthermore, we could correctly distinguish between individuals with active respiratory infections and those without infection with a 72.2 % classification accuracy. In some cases, it was also possible to differentiate between pathogens at the species level. These results are preliminary evidence to suggest the possibility of detecting infections in these patient without the use of time-consuming culture techniques.
Disciplines :
Chemistry
Author, co-author :
Stefanuto, Pierre-Hugues  ;  Université de Liège > Département de chimie (sciences) > Chimie analytique, organique et biologique
Rees, Christiaan A.;  Dartmouth College > Geisel School of Medicine
Romamno, Rosalba;  Imperial College of London > Department of Surgery and Cancer
Marczin, Nandor;  Imperial College of London > Department of Surgery and Cancer
Hill, Jane E.;  Dartmouth College > Thayer School of Engineering / Geisel School of Medicine
Language :
English
Title :
Identifying infectious agents from lung transplant patients using GC×GC-TOFMS
Publication date :
May 2017
Event name :
International Symposium on Capillary Chromatography and GCxGC symposium
Event date :
14-05-2017 to 19-05-2017
Audience :
International
Available on ORBi :
since 12 June 2017

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