Unpublished conference/Abstract (Scientific congresses and symposiums)
Identifying novel biomarkers for the rapid diagnosis of invasive aspergillosis using GC×GC-TOFMS
Stefanuto, Pierre-Hugues; Rees, Christiaan A.; Beattie, Sarah R. et al.
2017International Symposium on Capillary Chromatography and GCxGC symposium
 

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Keywords :
GCxGC; Volatile organic compounds; Aspergillus
Abstract :
[en] Aspergillus fumigatus is a filamentous fungus responsible for the life-threatening infection, invasive aspergillosis (IA), in immunocompromised individuals. Currently, IA diagnostic methods take days to produce a result and methods to obtain samples are invasive. One approach to improve the time-to-diagnosis is based on utilizing our knowledge of fungal metabolism during pathogenesis. Recent studies have demonstrated the importance of oxygen availability during IA, which presents a potential diagnostic opportunity. In this study, we analyzed the early (1 hr) and late (8 hr) volatile profile of A. fumigatus strain CEA1o under oxygen replete (~20% O2) and deplete (0.2% O2) conditions. In order to comprehensively screen the headspace of these cultures, we applied headspace solid phase microextraction (HS-SPME) associated with comprehensive two-dimensional gas chromatography coupled to time of flight mass spectrometry (GC×GC-TOFMS). Comparing the headspace of the four culture conditions, we were able to identify specific volatile biomarkers of low oxygen fungal growth: ethanol, acetaldehyde, 2-propanol, 2,3-butanedione, and 3-hydroxy-2-butanone. Based on the putative identifications of these compounds, we identified potential metabolic pathways that are responsible for their production. The two first compounds are known to be produced during hypoxia metabolism of glucose, however, the three others suggest that previously uncharacterized metabolic pathways may be utilized by A. fumigatus in low oxygen conditions. Validation of these biomarker identities and translation into patients may generate metabolic insight into these organisms during IA pathogenesis.
Disciplines :
Chemistry
Author, co-author :
Stefanuto, Pierre-Hugues  ;  Université de Liège > Département de chimie (sciences) > Chimie analytique, organique et biologique
Rees, Christiaan A.;  Dartmouth College > Geisel School of Medicine
Beattie, Sarah R.;  Dartmouth College > Geisel School of Medicine
Cramer, Robert A.;  Dartmouth College > Geisel School of Medicine
Hill, Jane E.;  Dartmouth College > Thayer School of Engineering / Geisel School of Medicine
Language :
English
Title :
Identifying novel biomarkers for the rapid diagnosis of invasive aspergillosis using GC×GC-TOFMS
Publication date :
May 2017
Event name :
International Symposium on Capillary Chromatography and GCxGC symposium
Event date :
14-05-2017 to 19-05-2017
Audience :
International
Available on ORBi :
since 12 June 2017

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