Diversity in sequences, post-translational modifications and expected pharmacological activities of toxins from four Conus species revealed by the combination of cutting-edge proteomics, transcriptomics and bioinformatics
[en] Venomous animals have developed a huge arsenal of reticulated peptides for defense and predation. Based on various scaffolds, they represent a colossal pharmacological diversity, making them top candidates for the development of innovative drugs. Instead of relying on the classical, low-throughput bioassay-guided approach to identify innovative bioactive peptides, this work exploits a recent paradigm to access to venom diversity. This strategy bypasses the classical approach by combining high-throughput transcriptomics, proteomics and bioinformatics cutting-edge technologies to generate reliable peptide sequences. The strategy employed to generate hundreds of reliable sequences from Conus venoms is deeply described. The study led to the discovery of (i) conotoxins that belong to known pharmacological families targeting various GPCRs or ion-gated channels, and (ii) new families of conotoxins, never described to date. It also focusses on the diversity of genes, sequences, folds, and PTM's provided by such species.
Disciplines :
Chemistry
Author, co-author :
Degueldre, Michel ✱; Laboratory of Mass Spectrometry, MolSys, ULg, Liege, Belgium
Verdenaud, Marion ✱; iBiTEc S/SPI Antibody Eng. for Health, CEA, Gif-sur-Yvette, France
Gilles, Nicolas; iBiTec-S, SIMOPRO, CEA, Gif-Sur-Yvette, France
Ducancel, Frédéric; iBiTEc S/SPI Antibody Eng. for Health, CEA, Gif-sur-Yvette, France
De Pauw, Edwin ; Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Quinton, Loïc ; Université de Liège > Département de chimie (sciences) > Chimie biologique
✱ These authors have contributed equally to this work.
Language :
English
Title :
Diversity in sequences, post-translational modifications and expected pharmacological activities of toxins from four Conus species revealed by the combination of cutting-edge proteomics, transcriptomics and bioinformatics
Publication date :
May 2017
Journal title :
Toxicon
ISSN :
0041-0101
eISSN :
1879-3150
Publisher :
Pergamon Press - An Imprint of Elsevier Science, Oxford, United Kingdom
FP7 - 278346 - VENOMICS - High-throughput peptidomics and transcriptomics of animal venoms for discovery of novel therapeutic peptides and innovative drug development
Funders :
DG RDT - Commission Européenne. Direction Générale de la Recherche et de l'Innovation [BE] CE - Commission Européenne [BE]
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