[en] Objective. Biocompatibility of polymer-infiltrated-ceramic-network (PICN) materials, a new
class of CAD–CAM composites, is poorly explored in the literature, in particular, no data are
available regarding Human Gingival Keratinocytes (HGK). The first objective of this study
was to evaluate the in vitro biocompatibility of PICNs with HGKs in comparison with other
materials typically used for implant prostheses. The second objective was to correlate results
with PICN monomer release and indirect cytotoxicity.
Methods. HGK attachment, proliferation and spreading on PICN, grade V titanium (Ti), yttrium
zirconia (Zi), lithium disilicate glass-ceramic (eM) and polytetrafluoroethylene (negative control)
discs were evaluated using a specific insert-based culture system. For PICN and eM
samples, monomer release in the culture medium was quantified by high performance liquid
chromatography and indirect cytotoxicity tests were performed.
Results. Ti and Zi exhibited the best results regarding HGK viability, number and coverage.
eM showed inferior results while PICN showed statistically similar results to eM but also to
Ti regarding cell number and to Ti and Zi regarding cell viability. No monomer release from
PICN discs was found, nor indirect cytotoxicity, as for eM.
Significance. The results confirmed the excellent behavior of Ti and Zi with gingival cells.
Even if polymer based, PICN materials exhibited intermediate results between Ti–Zi and eM.
These promising results could notably be explained by PICN high temperature–high pressure
(HT–HP) innovative polymerization mode, as confirmed by the absence of monomer release
and indirect cytotoxicity
Research center :
d‐BRU - Dental Biomaterials Research Unit - ULiège [BE]
Disciplines :
Dentistry & oral medicine
Author, co-author :
GRENADE, Charlotte ; Centre Hospitalier Universitaire de Liège - CHU > Service prothèse fixée
De Pauw-Gillet, Marie-Claire ; Université de Liège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
PIRARD, Catherine ; Centre Hospitalier Universitaire de Liège - CHU > Service de toxicologie
Bertrand, Virginie ; Université de Liège > Département de chimie (sciences) > Département de chimie (sciences)
Charlier, Corinne ; Université de Liège > Département de pharmacie > Chimie toxicologique
VAN HEUSDEN, Alain ; Centre Hospitalier Universitaire de Liège - CHU > Service prothèse fixée
Mainjot, Amélie ; Université de Liège > Département de sciences dentaires > Biomatériaux dentaires
Language :
English
Title :
Biocompatibility of polymer-infiltrated-ceramicnetwork (PICN) materials with Human Gingival Keratinocytes (HGKs)
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