Aptamers; HER2 receptor; 68Ga; PET imaging; bio-distribution; tumor-targeting
Abstract :
[en] Background: Two novel HER2 aptamers were recently selected with great potential for the in vitro diagnosis of HER2-positive cancer. The goal of this study was to examine the in vivo diagnostic potential of these HER2 aptamers.
Methods: Both HER2 aptamers were radiolabelled with 68Ga, injected in mice bearing a HER2-positive and HER2-negative tumour and evaluated by PET/MRI.
Results: Ex vivo bio distribution analysis revealed high uptake in the blood, tissues and organs, except the brain. Interestingly, this high uptake was explained by the slow blood clearance due to non-specific aptamer binding to blood proteins. We observed accumulation of radioactivity in both tumours in time. Although higher uptake in the HER2-positive tumour compared to the HER2-negative tumour was observed, this was accompanied with more necrosis in the HER2-negative tumour, which was observed by 18FDG PET/CT.
Conclusion: This work presents a first step towards the development of 68Ga-labelled aptamers for molecular cancer imaging.
Research center :
GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging & Memory - ULiège
Disciplines :
Radiology, nuclear medicine & imaging
Author, co-author :
Gijs, Marlies
Becker, Guillaume ; Université de Liège > Centre de recherches du cyclotron
Plenevaux, Alain ; Université de Liège > Département de chimie (sciences) > Département de chimie (sciences)
Bahri, Mohamed Ali ; Université de Liège > Centre de Recherches du Cyclotron > Centre de Recherches du Cyclotron
Aerts, An
Impens, Nathalie
Baatout, Sarah
Luxen, André ; Université de Liège > Département de chimie (sciences) > Laboratoire de chimie organique de synthèse
Language :
English
Title :
Biodistribution of Novel 68Ga-Radiolabelled HER2 Aptamers in Mice
Publication date :
11 August 2016
Journal title :
Journal of Nuclear Medicine and Radiation Therapy
eISSN :
2155-9619
Publisher :
OMICS International, Los Angeles, United States - California
