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See detailSensitivity of the Antarctic surface mass balance to oceanic perturbations
Kittel, Christoph ULiege; Amory, Charles ULiege; Agosta, Cécile ULiege et al

Poster (2017, December 15)

Regional climate models (RCMs) are suitable numerical tools to study the surface mass balance (SMB) of the wide polar ice sheets due to their high spatial resolution and polar-adapted physics. Nonetheless ... [more ▼]

Regional climate models (RCMs) are suitable numerical tools to study the surface mass balance (SMB) of the wide polar ice sheets due to their high spatial resolution and polar-adapted physics. Nonetheless, RCMs are driven at their boundaries and over the ocean by reanalysis or global climate model (GCM) products and are thus influenced by potential biases in these large-scale fields. These biases can be significant for both the atmosphere and the sea surface conditions (i.e. sea ice concentration and sea surface temperature). With the RCM MAR, a set of sensitivity experiments has been realized to assess the direct response of the SMB of the Antarctic ice sheet to oceanic perturbations. MAR is forced by ERA-Interim and anomalies based on mean GCM biases are introduced in sea surface conditions. Results show significant increases (decreases) of liquid and solid precipitation due to biases related to warm (cold) oceans. As precipitation is mainly caused by low-pressure systems that intrude into the continent and do not penetrate far inland, coastal areas are more sensitive than inland regions. Furthermore, warm ocean representative biases lead to anomalies as large as anomalies simulated by other RCMs or GCMs for the end of the 21st century. [less ▲]

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See detailObservation and simulation of ethane (C2H6) at 23 FTIR sites
Mahieu, Emmanuel ULiege; Franco, B; Pozzer, A et al

Poster (2017, December 13)

Ethane is the most abundant non-methane hydrocarbon (NMHC) in the Earth atmosphere. Its main sources are of anthropogenic origin, with globally 62% from leakage during production and transport of natural ... [more ▼]

Ethane is the most abundant non-methane hydrocarbon (NMHC) in the Earth atmosphere. Its main sources are of anthropogenic origin, with globally 62% from leakage during production and transport of natural gas, 20% from biofuel combustion and 18% from biomass burning. In the Southern hemisphere, anthropogenic emissions are lower which makes biomass burning emissions a more significant source. The main removal process is oxidation by the hydroxyl radical (OH), leading to a mean atmospheric lifetime of 2 months. Until recently, a prolonged decrease of its abundance has been documented, at rates of -1 to -2.7%/yr, with global emissions dropping from 14 to 11 Tg/yr over 1984-2010 owing to successful measures reducing fugitive emissions from its fossil fuel sources. However, subsequent investigations have reported on an upturn in the ethane trend, characterized by a sharp rise from about 2009 onwards. The ethane increase is attributed to the oil and natural gas production boom in North America, although significant changes in OH could also be at play.In the present contribution, we report the trend of ethane at 23 ground-based Fourier Transform Infrared (FTIR) sites spanning the 80ºN to 79ºS latitude range. Over 2010-2015, a significant ethane rise of 3-5%/yr is determined for most sites in the Northern Hemisphere, while for the Southern hemisphere the rates of changes are not significant at the 2-sigma uncertainty level. Dedicated model simulations by EMAC (ECHAM5/MESSy Atmospheric Chemistry; ~1.8×1.8 degrees) implementing various emission scenarios are included in order to support data interpretation. The usual underestimation of the NMHCs emissions in the main inventories is confirmed here for RCP85 (Representative Concentration Pathway Database v8.5). Scaling them by 1.5 is needed to capture the background levels of atmospheric ethane. Moreover, additional and significant emissions (~7 Tg over 2009-2015) are needed to capture the ethane rise in the Northern hemisphere. Attributing them to the oil and gas sector and locating them in North America allows EMAC to produce adequate trends in the Northern hemisphere, but not in the Southern hemisphere, where they are overestimated. Possible causes for this difference are discussed. [less ▲]

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See detailRole for PARs and thrombin-GPIbα interaction in thrombin induced procoagulant platelet formation
Bourcy, Morgane ULiege; Wang, Samuel; Pasalic, Leonardo et al

Poster (2017, December 11)

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See detailSystèmes d'informations foncières et politiques publiques (2015 - 2016)
Hendrickx, Sébastien ULiege; Ruelle, Christine ULiege; Zangl, Stéphanie ULiege et al

Poster (2017, December 11)

La recherche s’intéresse à la question de l’intérêt de développer l’observation foncière en Wallonie. Par une approche documentaire, sur la base d’entretiens auprès de témoins privilégiés et par une ... [more ▼]

La recherche s’intéresse à la question de l’intérêt de développer l’observation foncière en Wallonie. Par une approche documentaire, sur la base d’entretiens auprès de témoins privilégiés et par une enquête adressée à l’ensemble des communes wallonnes, elle tente d’évaluer la prise en compte des systèmes d’informations foncières en vue de mener à bien une politique d’aménagement du territoire efficace. Le poster présente les objectifs de cette recherche et les résultats acquis lors de la subvention 2015 - 2016. [less ▲]

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See detailSynthesis and Complexation of Sterically Hindered Imidazolium-2-dithiocarboxylate Ligands
Beltran Alvarez, Tomás Francisco ULiege; Zaragoza, Guillermo; Delaude, Lionel ULiege

Poster (2017, December 07)

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See detailThe Arabidopsis frd3 mutant phenotype: a new level of complexity
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Boulanger, Madeleine ULiege et al

Poster (2017, December 07)

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See detailDissociation entre recollection objective et subjective dans le vieillissement normal : une approche par analyses multiniveaux
Folville, Adrien ULiege; D'Argembeau, Arnaud ULiege; Bastin, Christine ULiege

Poster (2017, December 01)

Bien que le vieillissement normal soit associé à un déclin en recollection lorsque ce processus est mesuré de façon objective (mémoire de source ou rappel libre), l’expérience subjective de recollection ... [more ▼]

Bien que le vieillissement normal soit associé à un déclin en recollection lorsque ce processus est mesuré de façon objective (mémoire de source ou rappel libre), l’expérience subjective de recollection (vivacité du souvenir) demeure stable au cours du vieillissement, suggérant l’existence d’une dissociation entre ces deux processus (Mcdonough et al. 2014). A ce jour, les recherches ont toujours comparé ces deux aspects de la recollection séparément en mesurant les scores moyens de chaque participant, si bien que la relation entre mesures objective et subjective de recollection avec une approche essai-par-essai reste inconnue. Afin d’examiner cette question, nous avons recruté 34 participants jeunes et 34 participants âgés. Ils ont pris part à une tâche de recollection indicée au cours de laquelle ils mémorisaient des images complexes associées des titres descriptifs, chaque image étant présentée à gauche ou à droite de l’écran et avant ou après une pause. A la récupération, pour chaque essai, les participants devaient répondre à une question de mémoire de source spatiale ou temporelle et émettre un jugement de vivacité concernant leur souvenir de l’image associé au titre. Ensuite, les participants étaient invités à rappeler oralement autant d’éléments que possible concernant l’image. Des analyses multiniveaux ont été utilisées pour évaluer la relation entre les mesures de recollection subjective (vivacité) et objective (rappel libre) à travers les essais dans les deux groupes. Les résultats ont mis en évidence une différence significative entre les groupes en mémoire de source. Les participants âgés ont émis des jugements de vivacité plus élevés que les participants jeunes bien que leurs rappels libres soient moins détaillés, mettant ainsi en évidence une dissociation entre ces mesures. Les analyses multiniveaux ont révélé que la quantité de détail rappelé était un prédicteur significatif du jugement de vivacité dans les deux groupes, bien que cette relation soit plus importante chez les sujets jeunes par rapport aux âgés. Nos mesures de recollection objective suggèrent que le vieillissement normal diminue notre capacité à récupérer des informations contextuelles et des détails visuels associés à des souvenirs complexes. Les analyses multiniveaux suggèrent que les participants âgés ajusteraient moins bien leurs jugements subjectifs de vivacité vis-à-vis de la quantité d’information qu’ils récupèrent en mémoire comparé aux jeunes. Nous émettons l’hypothèse que des changements métacognitifs liés à l’âge ou qu’une utilisation d’informations différentes pour émettre un jugement de vivacité puisse expliquer cette faible correspondance entre recollection objective et subjective dans le vieillissement normal. [less ▲]

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See detailEvaluation of ground reaction forces based on kinematic data
Van Hulle, Romain ULiege; Schwartz, Cédric ULiege; Bruls, Olivier ULiege

Poster (2017, December 01)

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See detailElemental composition of soils and sediments by using portable X-ray fluorescence (pXRF) spectroscopy: a preliminary study
Alberti, Roberto; Falsone, Gloria; Gironda, M. et al

Poster (2017, December)

Portable X-ray fluorescence spectrometry (pXRF) provides rapid and low-cost analysis in laboratory and field conditions for a wide range of environmental applications. Its successful application in soils ... [more ▼]

Portable X-ray fluorescence spectrometry (pXRF) provides rapid and low-cost analysis in laboratory and field conditions for a wide range of environmental applications. Its successful application in soils requires a good instrument calibration and the possibility to implement custom calibrations and methods using known samples as well as reference materials. In the present study two set of samples, one from pedogenized sediments of a basin in Marradi (Italy; n=13) and the other from agricultural land in Meuse valley (Belgium; n=10), were analyzed by using a portable X-ray fluorescence (pXRF TRACER 5i made by Bruker) to detect and quantify the element species (Al, Si, Ca, Fe, Mg, K, Mn, P, S, Ba, Cd, Co, Cr, Cu, Mo, Pb, Sb, Sn, Sr and Zn). Element concentrations results were compared with analytical results from aqua regia extractions (AQ) followed by ICP-OES analysis. The aim of this work was to verify and find the best instrument setup for the specific matrix, elements and their concentration range with the purpose of using the pXRF technique directly insitu for the screening of sites potentially polluted. A standard factory calibration for soil applications was used for samples from Meuse valley, while a custom calibration for P, S, Ca and Fe quantification with the coefficient correction method was developed and used for pedogenized sediments from Marradi, as well as for Cu and Cr quantification in both sites. Comparing portable XRF and AQ results, elements could be grouped in three classes: 1) elements where the content determined by both methods is not correlated (p>0.05; Al, Si, and K); 2) elements strongly correlated (0.84<r<0.99, p<0.001; P, S, Mn, Fe, Ca, Cu, Cr, Zn and Pb); 3) elements with a weaker correlation (0.67<r<0.78, p<0.01; Mg and Sr). A further class of elements (Co, Mo, Cd, Sn, Sb, and Ba) was not evaluable because their concentration was below the detection limit of the pXRF. The measured concentrations and the relationship between pXRF and AQ were obviously affected by the elements form in the soils. The value of macroelements commonly related to the silicate phase measured by portable XRF is higher than that determined by AQ, which does not allow the destruction of Si-O and Al-O bounds (explaining the uncorrelation in the class 1 elements). The elements related to the organic matter (P and S, r2>0.60), carbonates (Ca, r2>0.55) and oxy(hydr)oxides (Fe and Mn) have instead comparable concentrations between pXRF and AQ. Preliminary results showed that portable XRF can quickly provide data for some elucidations of important pedogenic processes involving many elements (class 2) in the studied soils. Alternative methods offer results after expensive and time-consuming analytical procedures. Moreover for environmental and human health purposes, the pXRF should be a valid tool for a rapid screening of heavy metals, such as Cu, Cr, Zn and Pb. A larger set of soil samples and further custom calibration will be tested. [less ▲]

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See detailEco-extraction of aniseed oil: Use of green solvent as alternative solvent to n-hexane
Bettaieb ép Rebey, Ines ULiege; Bourgou, Soumaya; Detry, Pauline ULiege et al

Poster (2017, November 25)

Pimpinella anisum L. (anise) seeds have been widely used as a culinary ingredient as well as traditional remedies for the treatment of different disorders in the folk medicine systems of different ... [more ▼]

Pimpinella anisum L. (anise) seeds have been widely used as a culinary ingredient as well as traditional remedies for the treatment of different disorders in the folk medicine systems of different civilizations. Its essential oil show many properties and is already well studied and used. The lipid fraction of the seeds has also already been investigated. Petroselinic acid was the most prevalent fatty acid in anis oil seeds. This one also presents several properties. Nowadays, fixed oils are extracted with the help of petrosolvents. This raise issues regarding environment and security, but also public health. Regulations are more and more strict and alternative more and more searched. In general, those ones consist in the use of substitution solvents. Thus, the purpose of this work was designed to evaluate the performances of a green solvent compared to n-hexane in aniseed oil extraction. [less ▲]

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See detailRare Sulfamethoxazole Crystalluria – case report
Castiglione, Vincent ULiege; Diop, Coumba Souley ULiege; CAVALIER, Etienne ULiege et al

Poster (2017, November 24)

Case discussion We report here 7 Caucasian patients with very uncommon crystalluria. There were 3 female and 4 male of 54 to 86 years-old. Patients had no relevant medical record in common. However, they ... [more ▼]

Case discussion We report here 7 Caucasian patients with very uncommon crystalluria. There were 3 female and 4 male of 54 to 86 years-old. Patients had no relevant medical record in common. However, they all were hospitalized for different types of infections: three patients had urinary infection, two had osteitis, and the two last had sepsis. The patients had all been first treated with different antibiotherapy, which had then been replaced by cotrimoxazole after antibiogram. The administrated doses varied from 800mg to 4800mg a day of Sulfamethoxazole. Crystalluria description In all patients, the urine microscopic analysis revealed unusual crystals of various shapes including rectangles, thick parallelepipeds, truncated lozenges, spheroids, mushrooms, or “flowers”. Some crystals were incorrectly identified by the urinary sediment analyzer as uric acid, but we sought to determine them accurately. Most of the crystals were strongly birefringent and measured between 20 and 100µm. Urine pH varied from 5.0 to 6.5 on strip analysis. After urine centrifugation, we performed infrared spectrophotometry analysis on dried residue. In all cases, the infrared spectra allowed us to identify the N-acetyl-Sulfamethoxazole, the main metabolite of Sulfamethoxazole. Crystalluria was observed between 1 and 26 days after Sulfamethoxazole treatment initiation. The serum creatinine increased from 16% to 88% in 3 patients between the first day of Sulfamethoxazole treatment and the day of crystalluria. These considerations raised concern for drug implication in renal failure in some of these patients. Teaching points for the clinical condition Drug-induced kidney failure is well-known, but the direct precipitation of drug crystals into tubules is rare, and also probably under-evaluated. Even if Sulfamethoxazole tubular precipitation was probably not the main cause of renal failure in these cases, we suspect it could have played a role. N-Acteyl-Sulfamethoxazole can precipitate in urine in many uncommon crystals shapes that raise suspicion for drug nephrotoxicity. Automated urine analyzers may misidentify these rare crystals. Crystal’s recognition may be difficult even with polarized light microscopy. This is why they must be identified by infrared spectrophotometry to avoid misdiagnosis. These renal failures linked to Sulfamethoxazole precipitation are more susceptible to appear with high dosage of drug, hypovolemia and pre-existing renal failure. Hypoalbuminemia has also been described as a risk factor and was present in our four patients (between 26 to 39g/l, range laboratory: 43-54). Thus, prevention of Sulfamethoxazole precipitation consists in hydratation to maintain urine flow, and require adaptation of cotrimoxazole dosage in regard of renal function. Urine alkalinization (pH >7.0) is also possible in order to increase Sulfamethoxazole metabolite solubility. [less ▲]

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See detailTrophic ecology of icefishes (Notothenioidei, Perciformes) in a context of climate change
Peignot, Quentin; Frederich, Bruno ULiege; Lepoint, Gilles ULiege et al

Poster (2017, November 23)

The Southern Ocean currently undergoes major environmental modifications related to climate change. When faced with environmental changes, all organisms are expected to have some intrinsic capacity to ... [more ▼]

The Southern Ocean currently undergoes major environmental modifications related to climate change. When faced with environmental changes, all organisms are expected to have some intrinsic capacity to adapt their ecological habits to their new life conditions. Currently, biological traits of many Antarctic species are still unknown and we need such information to assess their capacity to adapt to natural and anthropogenic perturbations. Notothenioidei (Perciformes), or icefishes, are endemic to the Southern Ocean and they are an important trophic component of these marine ecosystems. Here we used stable isotopes ratios of carbon (δ13C) and nitrogen (δ15N) of two well represented genera of icefishes (Trematomus and Lepidonotothen) sampled in 2002/2004 and/or in 2015/2016 to better understand their resources partitioning and the influence of environmental changes on their trophic ecology. Isotopic niches (which are a proxy of the realized ecological niches) were modeled using the SIBER (Stable Isotope Bayesian Ellipses in R) R-package. Our results revealed a great variation in isotopic compositions among icefishes, suggesting that they exploit a wide array of resources. The overlap between the isotopic niches of Trematomus and Lepidonotothen genera in 2002/2004 highlights a possible past competition for resources. Moreover, temporal evolution of niches suggests taxon-specific ecological plasticity in response to variation in environmental parameters and/or in prey availability. An increase in resources partitioning between the two genera was observed over the studied period. In the future, it would be interesting to use mixing models including isotopic composition of potential preys to identify possible changes in their trophic ecology. [less ▲]

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See detailMorphological diversification of the endemic Antarctic fishes Trematominae (Notothenioidei, Teleostei)
Frederich, Bruno ULiege; Heindler, Franz Maximilian; Christiansen, Henrik et al

Poster (2017, November 23)

Adaptive radiation involves the early, rapid ecological and morphological diversification of multiple lineages from a common ancestor into new, diverging adaptive zones. Regarding this definition with ... [more ▼]

Adaptive radiation involves the early, rapid ecological and morphological diversification of multiple lineages from a common ancestor into new, diverging adaptive zones. Regarding this definition with emphasis on the tempo of diversification, Antarctic notothenioids represent one of the very few examples of adaptive radiation in marine fishes. Time-calibrated phylogenies suggest that the diversification of most speciose notothenioid lineages occured between 20 and 15 Ma. The subfamily Trematominae is one of those diverse subclades showing a large range of ecological niches. In the present study, we aim to describe the evolutionary history of Trematomus species. By combining a consensus time-tree and a geometric morphometric dataset, we first illustrate their pattern of cephalic shape diversification in a phylomorphospace and we infer the morphology of their common ancestor. We also explore whether the cephalic shape data shows a phylogenetic signal, which is defined as the statistical dependence among species trait values due to their phylogenetic relatedness. The combination of phylogenetic signal test and exploration of the phylomorphospace allows us to assess whether Trematomus rapidly diverged in various adaptive zones as expected under a classic scenario of adaptive radiation. [less ▲]

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See detailEffects of fluid preservation on sea star stable isotope compositions: How useful can museum collections be for trophic ecology studies?
Le Bourg, Baptiste ULiege; Lepoint, Gilles ULiege; Michel, Loïc

Poster (2017, November 23)

Stable isotope analyses (SIA) of carbon (δ13C) and nitrogen (δ15N) are a common tool to investigate the trophic ecology of animals. After field sampling, animal tissues are usually preserved dried or ... [more ▼]

Stable isotope analyses (SIA) of carbon (δ13C) and nitrogen (δ15N) are a common tool to investigate the trophic ecology of animals. After field sampling, animal tissues are usually preserved dried or frozen before subsequent analysis. However, museum samples, or samples not initially collected for SIA are often fixed in preservative solutions. These preservation methods may alter stable isotope ratios in animal tissues, and those effects should be quantified. Here, we investigated long-term effects (one year) of four preservation methods (freezing, alcohol, formaldehyde, drying) on δ13C and δ15N values of sea stars. We show that only formaldehyde preservation significantly affects δ13C values. However, after being altered, the mean δ13C value remained stable over time, suggesting that a correction factor may be used regardless of preservation time. δ15N values change across time for all methods except freezing but this may be the result of a higher intra-individual variability of δ15N values. The influence of preservation methods on estimation of isotopic niches size (parameterised as standard ellipse area) was also investigated. Bayesian estimations of standard ellipse areas (SEAB) of preserved samples were not different from those of fresh samples except for the samples preserved 6 months in alcohol. These preliminary results suggest that samples stored during one year in preservative fluids may be used for SIA in order to investigate the trophic ecology of sea stars. Future results will also help to determine whether the findings can be generalised to sea stars preserved for a longer time, such as those in museum collections. [less ▲]

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See detailAdductor mandibulae muscle and lower jaw morphology: an approach to determine bite strategies in piranhas and relatives (Serrasalmidae, Characiformes, Teleostei)
Huby, Alessia ULiege; Parmentier, Eric ULiege

Poster (2017, November 23)

Serrasalmidae (98 species) is a large monophyletic group of South American freshwater fishes. They have different dietary preferences ranging from the predominantly herbivorous or frugivorous pacus to the ... [more ▼]

Serrasalmidae (98 species) is a large monophyletic group of South American freshwater fishes. They have different dietary preferences ranging from the predominantly herbivorous or frugivorous pacus to the omnivorous or primarily carnivorous piranhas. Flesh-eating piranhas are supposed to have proportionally the most powerful bite forces among vertebrates but it has never been compared with those of plant, fruit and seed-eating species. Moreover, the bite strategy has never been explored in this family: is there a difference between carnivorous piranhas and herbivorous relatives? In the present study, we used the adductor mandibulae muscle and the lower jaw as models to answer this questioning through morphometric methods. We found that flesh-eating piranhas have a more developed adductor mandibulae muscle than seed-eating species which in turn have a larger jaw muscle than plant-eating species. The dorsomedial section of the jaw muscle (pars malaris) is the most developed in carnivorous species whereas it is the ventrolateral section (pars rictalis) in herbivorous species. We also distinguish two characteristic shapes of lower jaw that are related to dietary preferences. Results suggest different bite strategies: flesh-eating species possess a "scissor-like" way of feeding whereas the plant, fruit and seed-eating species show "vise-like" system. [less ▲]

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See detailA forward model for electrical conduction in soil-root continuum: a virtual rhizotron study
Rao, Sathyanarayan; Meunier, Félicien; Ehosioke, Solomon ULiege et al

Poster (2017, November 23)

Electrical Resistivity Tomography monitoring of soil-root system water fluxes have received growing interest in the past decades. Some studies suggest that roots can be more electrically conductive than ... [more ▼]

Electrical Resistivity Tomography monitoring of soil-root system water fluxes have received growing interest in the past decades. Some studies suggest that roots can be more electrically conductive than soil. We suggest that ERT data taken in agricultural fields is impacted by plant roots and might contaminate estimates of soil water content based on bare soil petrophysical relations. To understand how do roots impact electrical current flow and thus ERT data, a numerical electrical model was coupled with a mechanistic maize-soil water flow model. All the maize roots with a radius larger than 0.05 cm were explicitly accounted for in the finite element mesh and associated to their specific electrical properties. Root growth and water uptake processes continuously affected the EC contrast between soil and root. We demonstrated that high contrats between root and soil EC lead to errors in the estimation of soil water content, which could be disminished by using an appropriate biopedophysical correction term. The effective EC (bulk properties) of the medium computed using simulated plate electrodes at rhizotron boundaries reveal directional anisotropy induced by root processes and is more pronounced in sand medium when compared to loam. The percentage change in bulk EC due to change in direction (𝐸𝐸𝐸𝐸ℎ𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜 .vs. 𝐸𝐸𝐸𝐸𝑣𝑣𝑣𝑣𝑣 𝑣 𝑣𝑣𝑣𝑣𝑣 𝑣 ) starts at ~30 % in sand and ~3 % in loam when root is young and increases upto ~500% in sand and ~20% in loam at day 22 when root is three weeks old. Directions in which there is more anisotropy contains more information on the root processes and hence they can be used as prior information for ERT injection scheme to retrieve better information. [less ▲]

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See detailLes biais attentionnels envers l’alcool chez les « petits consommateurs » vs « gros consommateurs » dans un environnement virtuel
Pollet, Hélène; Quertemont, Etienne ULiege; Bouchard, Stéphane et al

Poster (2017, November 18)

Introduction : Les personnes dépendantes à l'alcool montrent des biais attentionnels quand elles sont confrontées à des indices liés à l’alcool (Kreusch, Vilenne, & Quertemont, 2013). D’après le modèle de ... [more ▼]

Introduction : Les personnes dépendantes à l'alcool montrent des biais attentionnels quand elles sont confrontées à des indices liés à l’alcool (Kreusch, Vilenne, & Quertemont, 2013). D’après le modèle de Wiers et al. (2007), les biais attentionnels favoriseraient la consommation répétée de substances. Objectif : Cette étude exploratoire évalue dans quelle mesure la réalité virtuelle fournit un environnement permettant de tester ces biais attentionnels. Hypothèse : Les gros consommateurs d'alcool présentent des biais attentionnels plus fréquents et plus intenses que les petits consommateurs. Méthode : Un questionnaire en ligne permettant de recruter 24 petits et 24 gros consommateurs d’alcool a d’abord été administré afin de présélectionner les sujets. Les participants ont ensuite réalisé une tâche d’immersion dans un environnement virtuel de « bar ». Le nombre de zooms effectués sur les stimuli « bières » et le nombre de commentaires rapportés en lien avec l’alcool étaient mesurés. Directement après l’immersion, la Visual Analogue Craving Scale (VAS) leur était administrée afin d’évaluer leur envie de boire. Résultats : Les « gros consommateurs » présentent davantage de zooms sur les stimuli « bières » que les «petits consommateurs». De plus, les « gros consommateurs » présentent un score plus élevé à la VAS que les «petits consommateurs », ce qui témoignerait d’une envie de consommer plus élevée après l’immersion par rapport aux « petits consommateurs ». Cependant, aucune corrélation significative n’a été mise en avant entre le nombre de zooms effectués et le score à la VAS chez les « gros consommateurs ». Conclusion : En accord avec nos hypothèses, nos résultats ont montré que les gros consommateurs présentent davantage de biais attentionnels envers les stimuli liés à l’alcool que les petits consommateurs ». Cependant, de futures recherches sont nécessaires afin de pouvoir confirmer que la réalité virtuelle fournit un environnement qui permet de tester les biais attentionnels. [less ▲]

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See detailFirm Performance and Multi-Scale Territorial Resources in Wallonia: Towards a Model using Business Accounting?
Wilmotte, Pierre-François ULiege; Halleux, Jean-Marie ULiege

Poster (2017, November 17)

In Europe (and in Wallonia), most public policies related to regional development and economic development aim to stimulate/reinforce the ‘’territorial resources’’ in order to improve the competitiveness ... [more ▼]

In Europe (and in Wallonia), most public policies related to regional development and economic development aim to stimulate/reinforce the ‘’territorial resources’’ in order to improve the competitiveness of the (Walloon, Belgian or European) firms. For instance, in Wallonia, one of the key action of the Marshall Plan (i.e. the main regional strategy in the domain of economic development) aims to “mobilize the territory” (in French, « mobilisation du territoire à destination du développement économique »). In this perspective, our objective is to quantify and analyse the territorial resources that impact the performance of Walloon or Belgian firms, with the final aim to advice the policy makers and to improve the related public policies. This topic is related to questions such as: Does a better accessibility of economic estates improve the performance of firms? Are cultural amenities (in order to attract talents) and land prices (in order to reduce their costs) strategic competitive advantages for firms? The poster describes some methodological issues of (in general) the PhD research and (particularly) one part of the research, concerning a multivariate statistics analysis of business accounting. [less ▲]

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See detailRapid changes in preoptic estradiol concentration during male sexual behavior
de Bournonville, Marie-Pierre ULiege; de Bournonville, Catherine; Ball, Gregory et al

Poster (2017, November 11)

Estrogens such as estradiol (E2) exert pleiotropic effects on physiological and behavioral responses such as neuroprotection, aggression or reproduction. Estrogens derived from local brain synthesis ... [more ▼]

Estrogens such as estradiol (E2) exert pleiotropic effects on physiological and behavioral responses such as neuroprotection, aggression or reproduction. Estrogens derived from local brain synthesis (neuroestrogens) are critical for the regulation of different functions including the control of male sexual behavior. Classically, E2 acts through effects initiated in the nucleus to regulate male sexual function. Along with these long-term effects, E2 also acts rapidly (within minutes) via membrane-initiated events. These effects are thought to depend on short-term variations in the local production of estrogens, through rapid fluctuations of the enzymatic activity of brain aromatase. In Japanese quail, rapid modulations of brain aromatase activity (AA) have been reported after sexual interactions or exposure to an acute stress. These changes take place mainly in the medial preoptic nucleus (POM), a sexually differentiated structure that plays a key role in the control of male sexual behavior and where aromatase is densely expressed. Yet, it has recently been shown that, in the short term, AA does not always reflect local E2 concentration. This study was designed to determine by in vivo microdialysis whether local E2 concentrations fluctuate during sexual interactions and test whether these changes parallel the decrease in AA observed ex vivo after copulation. We first conducted a series of experiments to validate the microdialysis and E2 assay. When dialysis probes were placed in successive baths containing known increasing amounts of E2, proportional changes in E2 concentration were measured in the dialysate. Moreover, a rise in E2 concentration was detected after in vivo retrodialysis of testosterone only if the probe was located within the POM and, after a peripheral injection of E2, a sharp rise of E2 was detected regardless of the probe location. Together these results show that in vivo microdialysis is a valid method to assess endogenous fluctuations of brain E2 concentrations in behaving animals. Two independent experiments then identified a rise in E2 concentrations in POM during sexual interactions. This increase occurred within 10 min after the initiation of the sexual interaction and was specific to the POM as there was no increase in E2 concentrations in males that had their cannula outside of this area. Together these data confirm that rapid changes in AA measured ex vivo cannot be considered as a reliable proxy for E2 concentrations. The discrepancies could originate either from the different time resolution related to the two techniques or from differences in the microenvironment in which aromatase functions in vivo and during ex vivo assays. [less ▲]

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See detailImplementation of "Parler-Bambin" preventive program in the multilingual and multicultural context of nurseries/daycares in Lebanon
Moitel ép Messarra, Camille ULiege; Maillart, Christelle ULiege

Poster (2017, November 10)

This project is targeting prevention programs for language and communication difficulties in nurseries in Lebanon. More specifically, it will explore the implementation of a preventive program to enhance ... [more ▼]

This project is targeting prevention programs for language and communication difficulties in nurseries in Lebanon. More specifically, it will explore the implementation of a preventive program to enhance language skills in early childhood, and to specify the role of the Speech and Language Therapist (SLT) in this implementation. There is a growing consensus among researchers and clinicians that abilities acquired at an early age, especially in oral language, are predictive of children's academic performance and future social integration (Zorman, 2011). Preventive actions are increasingly recognized as a priority to improve public health worldwide (Law et al., 2013). In Lebanon, an ongoing national program (MHPSS 2015-2021 - MHPSS, Preventive and Curative, Ministry of Public Health, 2015) is taking part of this kind of healthcare development. Aims: Thus, taking into account some variables that affect the implementation process (environment communities, providers, prevention delivery system and the prevention support system), the aims of this project are 1) to study the feasibility of a preventive program model, that partners Speech and Language Therapist and educators or caregivers in daycare and nurseries, to facilitate language and communication in Lebanese toddlers (under 3 years old), 2) determine the success factors of the implementation. It will be based on a French program “Parler Bambin”, which is currently widely developed in France. Method: The first study will focus on the factors of engagement of different stakeholders in a prevention program: firstly, by identifying the professionals involved in nurseries in Lebanon and then identifying the characteristics of these stakeholders in diverse childcares. Data will be gathered through a questionnaire constructed or adapted from existing questionnaires for this purpose (eg, Preschool Teacher Literacy Beliefs Questionnaire [TBQ], Hindman and Wasik, 2008). Similarly, the questionnaires will address SLT’s representations about their role in supporting language in nurseries. Nominal groups or focus groups (Baribeau and Germain, 2010) and questionnaires will be also carried out to identify the facilitators of this kind of preventive actions and the specific needs of SLTs in preventive settings. A second phase will target the implementation process itself: adapting and implementing a preventive intervention, while taking into consideration implementation aspects. We will find out major variables that are worthy for the program’s implementation, by measuring its fidelity (dosage, quantity, intervention strength, quality, participant responsiveness, program differentiation, etc.), also using videos and direct coaching sessions. Pre and posttest measures will be applied on children (MCDI -The MacArthur communicative development inventories, Fenson et al., 1996; Questionnaire for parents of bilingual children LITMUS-PABIQ, COST IS0804, validation in Lebanon, Tuller 2015). Finally, following the pilot study, the implementation of the adapted program will be proposed according to different execution modalities to support its implementation: with a direct or indirect involvement of an SLT. It will be tested more widely, with particular focus on the role of the speech therapist. Evaluation measures similar to those presented in Phase 2 will be carried out. Expected results: This project will highlight the added value of speech therapy in setting up a prevention program. It also participates in the elaboration of a national strategic framework for the Speech and Language Therapy [less ▲]

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See detailTeacher-SLT co-intervention program to support language in kindergartens in Lebanon: methodology, procedures and measures
El Kouba, Edith ULiege; Maillart, Christelle ULiege

Poster (2017, November 10)

Background: The aim of this project is to develop a co-intervention program, lead by a teacher and an SLT, to support oral language in preschoolers in the Lebanese context (multicultural, multilingual ... [more ▼]

Background: The aim of this project is to develop a co-intervention program, lead by a teacher and an SLT, to support oral language in preschoolers in the Lebanese context (multicultural, multilingual). Actually, there is a growing consensus, that efforts to enhance children’s early language skills, can improve their reading skills and long-term academic achievement. However, some children are at risk of delays due to diverse environments (e.g. low socioeconomic status), and impoverished language and literacy experiences. Thus, international recommendations highlight the necessity for SLTs to conceptualize their intervention, according to community based needs, focusing on primary prevention for early communication and language difficulties, given their later impact on social and professional integration (Law 2013). Aims: In line with the recommendations of the Ministry of Public Health in Lebanon (Mental Health program services MHPSS, preventive and curative, Ministry of Public Health, 2015), the purpose of the project is to support language skills in preschoolers by adapting, implementing and analyzing co-intervention program that partners early childhood educators along with SLTs. Methods: First, we will identify the beliefs, representations and expectations of the different stakeholders (teachers, speech-language pathologists, school principals) through questionnaires and interviews among the diverse Lebanese school contexts in order to 1) identify the knowledge and 2) gather their expectations in the area of support and prevention for language difficulties in kindergarten. Second, the project aims to identify the quality of teacher-child interaction, considered as one of the primary mechanism that foster the development of child’s language competences. It is commonly assessed by using the Classroom Assessment Scoring System (CLASS-K; Pianta, La Paro, & Hamre, 2008). Ten dimensions will be considered: climate (positive or negative), teacher sensitivity, children's perspectives, behavior management, productivity, teaching practices and learning formats, concept Development, quality of feedback provided to the child, and linguistic modeling. Finally, we will design a co-intervention program that is the most consistent with the contextual, cultural and linguistic diversity in Lebanon. Two intervention settings will be addressed and compared: 1) a co-intervention with an SLT including direct coaching, and video-retroactions and 2) an implementation of the program led by the teacher after a training provided by an SLT. We will collect information about children’s bilingual environment through the Parental Bilingual Questionnaire (PABIQ-LITMUS, COST Action IS804), and we will evaluate interactions through CLASS-K (Pianta, La Paro, & Hamre, 2008), pre and post evaluation. Children's language skills will be measured through: ELO-L (Zebib, Khomsi, Henry, Messarra, & Kouba Hreich, Lebanon Tests Editions, 2017). Moreover, measurements of the fidelity of program’s implementation (dosage, adhesion, quality of the interactions, responsiveness) will also be carried out. Results: The expected results are intended to demonstrate to which extent an intervention program, to enhance communication and language skills, carried out jointly by teachers and speech therapists, would be more effective than a program delivered without the intervention of SLTs. The intervention of speech-language pathologists would thus have a more effective impact on the teachers' practices and the language skills of the children. This would justify the primary role of SLTs in indirect interventions in preschools in Lebanon. [less ▲]

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See detailInjection of spin-polarized current in a Ge-based magnetic device with coplanar contacts
Fourneau, Emile ULiege; Avila Osses, Jonathan ULiege; Silhanek, Alejandro ULiege et al

Poster (2017, November 09)

Electron spin injection and spin detection in magnetic materials are key features to the functionalization of electron spin polarization as a degree of freedom for both information processing and storage ... [more ▼]

Electron spin injection and spin detection in magnetic materials are key features to the functionalization of electron spin polarization as a degree of freedom for both information processing and storage. Currently, spin-dependent tunneling in magnetic junction devices is the most common approach to achieve efficient spin injection. However, many recent studies highlighted the interesting possibility to create spin-polarized currents in structures which combine a magnetic semiconductor, e.g. magnetic alloys based on group-IV semiconductors or diluted magnetic semiconductor compounds, forming a Schottky-like rectifying junction with a metallic ferromagnet. Although theoretical works have already addressed the performance of this structure by numerical simulations of the spin drift and spin diffusion equations, taking into account various characteristics of the ferromagnet (FM) / semiconductor (SC) interface such as barrier height and boundary roughness in 1D models, correlations with experimental results are scarce. This work aims at achieving spin injection with Ge-based magnetic structures using a rectifying junction in a coplanar architecture. We performed 2D numerical calculations of the spin drift and diffusion process in the direct neighborhood of a junction consisting of a Mn5Ge3 half-metallic ferromagnet acting as the injecting contact and an n-type Ge film, forming 3- and 4-terminal devices. Our results show that geometrical effects play a major role on the spin injection efficiency. Moreover, the simulations emphasize the asymmetry of spin accumulation at the FM/SC interface as well as a strong effect of the depletion layer caused by the Schottky contact junction. [less ▲]

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See detailPE teachers as promotors of physically educated citizens: Comparison of their representations in Quebec and Wallonia
Cloes, Marc ULiege; Carbonneau, Ulysse; Grenier, Johanne

Poster (2017, November 08)

If physical education (PE) was sport oriented since the 80’s, an international movement underlined a need of change in order to deal more with the society evolution. Since the turn of the new millennium ... [more ▼]

If physical education (PE) was sport oriented since the 80’s, an international movement underlined a need of change in order to deal more with the society evolution. Since the turn of the new millennium, PE has been increasingly linked to the promotion of physically active lifestyles. The implementation of such change of core values differed dramatically from one country to another. For example, in two French speaking regions, Quebec and Wallonia, the educational stakeholders reacted in different ways. As soon as 2001, the Quebec Ministry of Education decided to entrust PE and health education to PE teachers while the Wallonian educational authorities seem to discover today the central role that PE teachers could play in promoting a healthy lifestyle in youth. In this study, we compared Quebec and Wallonian PE teachers’ representations of their role as promotors of an active lifestyle. Six Canadian (3 females) and 11 Wallonian PE teachers (6 females) answered to a semi-structured interview. It was focused on: students’ motivation and self-confidence to engage in regular physical activity; knowledge about the health benefits of an active lifestyle, and self-determination to practice regular physical activity. A content analysis of the verbatim was processed in order to identify the main concepts expressed by the subjects. At the current state of the data analysis, it appears that cultural differences are clearly identified between Canadian and Wallonian PE teachers. The development of a healthy active lifestyle is more predominant among Canadian educators who can draw their experiences in more than 10 years of the Physical and Health Education curriculum while their colleagues from Wallonia are still trying to test new approaches without a strong institutional support. Wallonian teachers could take benefit of their colleagues’ experience in order to facilitate the adoption of news teaching strategies. [less ▲]

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See detailModulation of NMDA receptor mediated excitatory synaptic currents in dopamine neurons of the substantia nigra.
Destreel, Geoffrey ULiege; Seutin, Vincent ULiege; Engel, Dominique ULiege

Poster (2017, November 08)

Dopamine (DA) neurons of the substantia nigra pars compacta (SNc) exhibit two main firing modes, spontaneous single action potential (AP) firing and bursting. The amount of DA released by these neurons in ... [more ▼]

Dopamine (DA) neurons of the substantia nigra pars compacta (SNc) exhibit two main firing modes, spontaneous single action potential (AP) firing and bursting. The amount of DA released by these neurons in target areas depends on the presynaptic AP firing pattern and is essential to modulate several aspects of behavior such as the control of movement. Bursting activity is mediated by excitatory afferents and specifically by the activation of NMDA receptors (NMDARs). However, the level of activation of NMDARs at these synapses during spontaneous synaptic activity is unknown. We assessed the occupancy of the glycine binding sites of the NMDAR by testing the effects of coagonists and by blocking the uptake of glycine on spontaneous excitatory postsynaptic currents (sEPSCs) in DA neurons of the SNc. [less ▲]

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See detailRaman Chemical Imaging in Kidney Stone Analysis
Castiglione, Vincent ULiege; Sacre, Pierre-Yves ULiege; CAVALIER, Etienne ULiege et al

Poster (2017, November 02)

Background: The structure of kidney stones might provide clinical useful information in addition to the stone composition. The Raman chemical imaging (RCI) is a new technology used for the production of ... [more ▼]

Background: The structure of kidney stones might provide clinical useful information in addition to the stone composition. The Raman chemical imaging (RCI) is a new technology used for the production of two-dimensions maps of the constituents' distribution in samples. We aimed at determining the use of RCI in urinary stone analysis. Methods: Twelve calculi were analyzed by RCI using a confocal Raman microspectrophotometer. They were selected according to their heterogeneous composition and morphology. Prior to the analysis, samples were sliced and milled in order to detect the nucleus of the stones and having a smooth surface. RCI was performed on the whole section of stones. Once acquired, the data were baseline corrected and analyzed by MCR-ALS. Results were then compared to the spectra obtained by Fourier Transform Infrared spectroscopy, the gold standard method for the determination of urolithiasis composition. Results: RCI succeeded in identifying all the chemical components contained in each sample, including monohydrate and dihydrate calcium oxalate, anhydrous and dihydrate uric acid, apatite, struvite, brushite, whitlockite and ammonium urate. However, proteins couldn't be detected because of the huge autofluorescence background and the small concentration of these poor Raman scatterers. Carbapatite and calcium oxalate were correctly detected even when they represented less than 5 percent of the whole stones, allowing the detection of very small structures like Randall's plaques. Moreover, RCI provided the distribution of components within the stones. The nuclei were accurately identified, as well as thin layers of other components. Conversion of dihydrate to monohydrate calcium oxalate was correctly observed in the center of one sample. Conclusion: RCI showed a good accuracy in comparison with infrared spectroscopy in identifying components of kidney stones. In addition, RCI is nondestructive enabling the storage of samples. This analysis was also useful in determining the organization of components within stones, which help locating constituents in low quantity, such as nuclei. However, this analysis is time-consuming, which makes it more suitable for research studies rather than routine analysis. [less ▲]

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See detailComparison of faecal microbiota of horses suffering from atypical myopathy and healthy co-grazers
Cerri, Simona ULiege; Taminiau, Bernard ULiege; Votion, Dominique ULiege et al

Poster (2017, November 02)

Objectives: To characterize faecal microbiota of horses with atypical myopathy (AM) compared with healthy co-grazers (HcG). Methods: Fresh faecal samples were obtained from 6 horses (1 stallion, 3 ... [more ▼]

Objectives: To characterize faecal microbiota of horses with atypical myopathy (AM) compared with healthy co-grazers (HcG). Methods: Fresh faecal samples were obtained from 6 horses (1 stallion, 3 geldings and 2 females; mean age of 11.810 years) with confirmed AM and 6 HcG (4 geldings and 2 females; mean age of 13.68 years) during autumn-2016 and spring-2017 AM outbreaks in Belgium. Bacterial taxonomy profiling obtained by 16S amplicon sequencing of faeces was used to identify differentially distributed bacterial taxa between AM and HcG. Results were statistically compared using Welch's t-test with STAMP software. Results: A total of 90,407 sequences were analysed and clustered to 8,066 operational taxonomic units. Bacterial populations were distributed between 17 phylas, although 20% of sequences could not be attributed to an existing phylum. Horses with AM harboured a significantly higher relative abundance of Ruminococcaeae family with a significantly lower Lachnospiraceae when compared to HcG. Discussion: AM is caused by hypoglycin A intoxication, but only a part of horses pasturing in the same toxic environment develops the pathology, suggesting that there may be protective factors at the horse level. The results of this study show significant differences in faecal microbiota between AM cases and HcG, which could suggest that microbiota could play a role in the development or prevention of clinical disease. Conclusions: Results demonstrate that microbiota of AM affected horses is significantly different compared to HcG. Significance: Microbiome could influence the development of AM, but this role deserves further investigation. [less ▲]

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See detailEssay of Siganus sutor aquaculture in Madagascar: for promising alternative activity for traditional fishermen
Ravelohasina, Helga; Rasolofonirina, R.; Mahafina, J. et al

Poster (2017, November)

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See detailCoalescence in Highly Viscous System
Leleu, David ULiege; Pfennig, Andreas ULiege; Bruns, Nicole

Poster (2017, November)

study of the coalescence in liquid-liquid dispersion by comparing batch settling cell experiment with a numerical tool, based on ReDrop concept.

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See detailPlant phenotyping activities at the Walloon Agricultural Research Centre
Vincke, Damien; Durenne, Bastien ULiege; Mingeot, Dominique et al

Poster (2017, November)

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See detailUsing phenotyping and complementary VOC profiling approach to investigate physiological response of Brassica napus L. plantlets under cadmium and epoxiconazole abiotic stresses
Durenne, Bastien ULiege; Geerts, Pascal; Druart, Philippe et al

Poster (2017, November)

Thanks to a laboratory, high-throughput and sterile homemade glass chambers system giving us the capacity to follow VOC (Volatile Organic Compounds) emissions at any time during plant growth, we are able ... [more ▼]

Thanks to a laboratory, high-throughput and sterile homemade glass chambers system giving us the capacity to follow VOC (Volatile Organic Compounds) emissions at any time during plant growth, we are able to compare the phenotype of the plant and its VOC emission in the same time. Although the system could be used for biotic stress too, we decided to investigate the physiological response of oilseed rape plantlets such as model plant concerning two atypical abiotic stresses: cadmium and epoxiconazole. Experiments were achieved under several concentrations corresponding to a gradient from a tolerant to a sublethal dose. Oilseed rape plantlets phenotyping consisted in daily symptoms observation (chlorosis for cadmium stress and shorter internodes length for expoxiconazole stress), shoots and roots length recording and finally, fresh and dry weight assessment. The main goal was therefore to analyse VOC profile with a non-destructive method in parallel to this phenotype characterization in absence of interferences with uncontrolled environmental conditions. Cadmium was chosen because it can be problematic within agricultural soils (phosphate fertilizers among other things) and epoxiconazole because it is a systemic fungicide used in cereals known to be hugely persistent into agricultural soils. [less ▲]

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See detailBIGMOMAL - Big Data Analytics for Mobile Malware Detection
Wassermann, Sarah ULiege; Casas, Pedro

Poster (2017, November)

Mobile malware is on the rise. Due to their popularity, smartphones represent an attractive target for cybercriminals, especially regarding unauthorized access to private user data; smartphones ... [more ▼]

Mobile malware is on the rise. Due to their popularity, smartphones represent an attractive target for cybercriminals, especially regarding unauthorized access to private user data; smartphones incorporate a lot of sensitive information about users, even more than a personal computer. Indeed, besides personal information such as documents, accounts, passwords, contacts, etc., smartphone sensors centralize other sensitive data such as user location, physical activities, etc. In this paper, we study the problem of malware detection in smartphones, using supervised machine learning models and big data analytics frameworks. Using a publicly available dataset for smartphone data analysis (the SherLock data collection, see http://bigdata.ise.bgu.ac.il/sherlock/), we train and benchmark different supervised machine learning models to detect malware apps activity.The Sherlock data collection is a crowdsourcing-based smartphone dataset in which hundreds of features from many different "sensors" or vantage points within the device are monitored, using a tailored smartphone agent. The collection is done during a long-term - 2 years (2015/16), field trial on 50 smartphones used as primary device for 50 different participants. The monitoring agent collects a wide variety of network, software and sensor data at a high sample rate (as low as 5 seconds); in addition, participant devices include a sandbox-like smartphone agent which runs controlled malware apps, perpetrating attacks on the user's device (such as contacts theft, spyware, phishing, etc.), while creating labels for the SherLock dataset. The complete labeled dataset contains more than 10 billion data records, with a total of about 4 TB of data. We additionally complement the labels for malicious apps which might have been installed by participants by analyzing the installed apps' hashes in Virus Total (https://www.virustotal.com), a well-known multi antivirus online scanning system. From the complete dataset, we keep two specific feature categories: all those features related to the network traffic generated by the apps, and all those features corresponding to the footprint of the app on the CPU and internal running processes (e.g., statistics on CPUs, memory usage, linux-level processes information, etc.). The rationale is that some malware activity would be more visible at the network traffic level, whereas some others would be better identified at the local processes level. Using this dataset, we train different machine learning models (e.g., decision trees, neural networks, SVMs, etc.) and verify their accuracy to automatically spot out malicious apps running on the users’ devices. We also apply feature selection strategies to improve results and reduce computational times. Given the size of the dataset, we rely on big data platforms (such as Spark) to perform the analysis, complementing the machine learning based analysis with scikit-learn like pipelines. We evaluate three different concepts, including (i) overall model performance (using multi-fold cross validation on the complete dataset), (ii) generalization of the learned models across different users (train in N-1 users, and test in the remaining user), and (iii) detection accuracy drift along time (train during first month/week, test the resulting model in the subsequent months/weeks). Initial results are very promising, especially regarding overall model performance for decision tree based models. [less ▲]

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See detailAnycast on the Move - A First Look at Mobile Anycast Performance
Wassermann, Sarah ULiege; Rula, John P.; Bustamante, Fabian

Poster (2017, November)

Service providers rely on replication to improve service performance and reliability, placing instances in multiple locations and redirecting clients to nearby copies. Anycast is a common mechanism used ... [more ▼]

Service providers rely on replication to improve service performance and reliability, placing instances in multiple locations and redirecting clients to nearby copies. Anycast is a common mechanism used for redirecting clients in a variety of domains from naming to CDNs and video streaming. IP anycast offers a method for making a service IP address available to a routing system from several locations at once, and clients' requests are directed based on BGP routing policies. For operators, IP anycast offers an economic, scalable, and simple approach to replicated services; BGP provides considerable robustness, adapting to changes in service and network availability. For clients, however, the mapping can be suboptimal, unstable, and seemingly chaotic, as routing policies have not only technical motivations, and routing changes can silently shift traffic from one site to another with a consequent loss of shared state and potential performance impact. Given its wide deployment and interesting tradeoffs, IP anycast has been the focus of much recent measurement work. All prior studies have, nevertheless, focused on wired networks despite the growing dominance of mobile Internet. Today, the number of mobile subscriptions is over 7.4 billion, and users spend over 2x times more hours browsing on their smartphones than on any other device, with the corresponding increase on cellular traffic. We present early results on the first study of anycast performance for mobile users. Our evaluation focuses on two distinct anycast services, K-and F-Root, each providing part the DNS Root zone. Both services are widely replicated with publicly available site locations and unicast IP addresses that allow us to evaluate the relative performance of anycast routing to its "optimal" (in terms of unicast) site location. We collected active measurements from geographically distributed clients on both cellular and WiFi networks from September 2016 until April 2017, using the Aqualab’s ALICE engine [1]. In each experiment clients launched ping and traceroute measurements towards the root servers’ anycast addresses, as well as to five chosen unicast addresses determined to be the closest to the client in terms of geographic distance, at an hourly rate. Clients also recorded their geographic location, anonymized to a 10 km² area. Our findings show that mobile clients are routed to suboptimal replicas in terms of geographical distance, more frequently while on a cellular connection than on WiFi, with a significant impact on perceived service performance. The phenomenon seems to be more pronounced for K-Root than for F-Root. A possible explanation for the long distances would be that our cellular clients are simply far away from all the available replicas. However, our investigations demonstrate that this is not necessarily the case. Finally, we start to explore the root causes for anycast anomalies in cellular networks. We reveal three classes of anomalies: distant client packet gateways, poor anycast routing within Tier-1 networks, and improper routing out of cellular networks. [1] http://aqualab.cs.northwestern.edu/projects/261-alice [less ▲]

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See detailENTOFÔR project -From waste to resource
Lecrenier, Marie-Caroline ULiege; Fumière, Olivier; Berntssen, M.H.G. et al

Poster (2017, November)

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See detailIs oral feeding compatible with an unresponsive wakefulness syndrome?
MELOTTE, Evelyne ULiege

Poster (2017, November)

Introduction : Vegetative state/unresponsive wakefulness syndrome (VS/UWS) is defined by the presence of eye-opening and the absence of awareness and voluntary movement [1]. VS/UWS patients classically ... [more ▼]

Introduction : Vegetative state/unresponsive wakefulness syndrome (VS/UWS) is defined by the presence of eye-opening and the absence of awareness and voluntary movement [1]. VS/UWS patients classically receive hydration and nutrition through an enteral feeding tube. Objective - Methods : We retrospectively reviewed the clinical information of 65 VS/UWS patients (aged 45±12; range 16-85 years) evaluated at the CHU hospital of liege searching for mention of oral feeding. VS/UWS diagnosis was made after repeated behavioral assessments using the standardized coma recovery scale–revised (CRS-R, [2]) in association with complementary evaluations using neuroimaging techniques. Results : Of the 65 VS/UWS patients, two could resume oral feeding (3%). One could achieve full oral feeding and the other had oral feeding in addition to gastrostomy feeding. Neuroimaging evaluations showed in both patients a massive decrease in the spontaneous brain activity and its functional connectivity (using functional magnetic resonance imaging), bilateral cerebral cortex hypometabolism and preserved metabolism in the brainstem and cerebellum (using positron emission tomography). Discussion – Conclusion – Clinical message : oral feeding is rare in VS/UWS patients (3% in our cohort). Based on neuroimaging results, this behaviour does not seem to be incompatible with the diagnosis of VS/UWS but the neuromecanistic root, which allows this behavior, still needs to be elucidate. This study also emphasizes the importance of assess and manage deglutition in patients with altered state of consciousness regardless of their level of consciousness. Tactile oro-facial stimulation, manual therapy, taste stimulation and therapeutic feeding can be another “gateway” to interact with these patients and improve their quality of life. [less ▲]

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See detailCarbon and nutrients sources and transformation in the River Sabaki, Kenya
Tamooh, FT; Shawlet, C; Borges, Alberto ULiege et al

Poster (2017, October 30)

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See detailLa β-alanine dans des épreuves isocinétiques et de sauts répétés, (in)utile?
Paulus, Julien ULiege; Schwartz, Cédric ULiege; Paquot, Nicolas ULiege et al

Poster (2017, October 30)

Introduction La β-alanine (β-A), acide aminé précurseur de la carnosine, a fait l'objet d'un nombre important d'études sans néanmoins parvenir à un consensus quant à son influence sur la performance [1-6 ... [more ▼]

Introduction La β-alanine (β-A), acide aminé précurseur de la carnosine, a fait l'objet d'un nombre important d'études sans néanmoins parvenir à un consensus quant à son influence sur la performance [1-6] et/ou son mécanisme d'action [7]. Notre étude a pour but d'affiner la connaissance de son impact sur des performances maximales lors d'épreuves isocinétiques et de sauts prolongées: les personnes ayant une faible résistance à la fatigue neuromusculaire bénéficieraient-elles davantage des effets de la β-A? Méthodes Neuf hommes (24,5 ± 1,2 ans, 182,1 ± 6,6 cm, 80,2 ± 9,9 kg), ont réalisé deux épreuves d'exploration de la fatigue neuromusculaire avec 48h à 72h de repos entre chaque: un test analytique mono-articulaire, gold standard de l'évaluation musculaire, et une épreuve poly-articulaire dite "fonctionnelle". Ces deux épreuves, complémentaires de par les informations qu'elles permettent d'obtenir, sont respectivement un test isocinétique de résistance à la fatigue (30 extensions/flexions maximales de genou en concentrique à 180°.s-1 sur une amplitude de 100° sur Cybex Humac CSMI) [8] et un test de countermovement jump répétés (35 sauts maximaux enclenchés toutes les 1,82 secondes). Chaque sujet a réalisé quatre fois chaque testing: avant/après 14 jours de supplémentation en β-A (5g/j.) et avant/après 14 jours de prise d'un placebo (lactose) sous forme d'un crossover randomisé en double aveugle avec un wash-out de 14 jours. Résultats Aucun effet global de la supplémentation en β-A n'a été observé, que ce soit pour l'épreuve isocinétique (entre autres, somme du travail total des extenseurs: ES Cohen = 0,06 [CI95%: -0,57/0,68]; Magnitude-Based Inference (MBI) Hopkins: P (positif) 31% / T (trivial) 51% / N (négatif) 18%) ou de sauts répétés (entre autres, somme des hauteurs des 35 sauts: ES Cohen = -0,09 [CI95%: -0,47/0,28]; MBI: P 5% / T 68% / N 26%). Une corrélation négative (inversement proportionnelle), forte et statistiquement significative a néanmoins été observée entre l'impact de la β-A sur la performance et la capacité de résistance à la fatigue neuromusculaire pour l'épreuve isocinétique (entre autres, pente de la régression linéaire du travail total & différence entre somme du travail total des extenseurs avec β-A et placebo: rPearson = - 0,85 [CI95%: -0,97/-0,44] avec une p-value de 0,002). Pour l'épreuve de sauts répétés, les résultats ne sont pas significatifs (rPearson = - 0,31 [CI95%: -0,81/0,44] avec une p-value de 0,409). [less ▲]

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See detailUpscaling winter wheat above-ground biomass measurements using multispectral imagery and 3D data from unmanned aerial vehicle
Michez, Adrien ULiege; Bauwens, Sébastien ULiege; Heinesch, Bernard ULiege et al

Poster (2017, October 20)

Field measurements in the ICOS program are spatially limited whereas the monitored gas fluxes may have a large footprint. Aerial remote sensing has the advantage to monitor large areas. The main goal of ... [more ▼]

Field measurements in the ICOS program are spatially limited whereas the monitored gas fluxes may have a large footprint. Aerial remote sensing has the advantage to monitor large areas. The main goal of our research was to test the potential of unmanned aerial vehicles (UAV) to upscale parameters monitored through the ICOS program. In this study, we specifically focus on above-ground biomass (AGB) monitoring in a winter wheat crop. We used a octocopter drone (X frame type) to acquire a time series over the crop growing season (8 flights from the 14th of February 2017 to the 7th of July 2017) of multispectral imagery covering the ICOS candidate station of Lonzée (Wallonia, Belgium) and the surrounding field crop areas (ca. 0.25 km² per flight). The multispectral camera provides spectral information on the green (550 +/- 50 nm), red (660 +/- 50 nm), near infrared (735 +/- 50 nm) and red-edge (790 +/- 10 nm) wavelengths bands. The UAV also brought an off-the-shelf high resolution (20 Mpx) RGB camera to derive accurate 3D data. We performed a photogrammetric 3D reconstruction of the acquired imagery for every flight survey. The images provided by the RGB sensor (Sony RX100) were used to produce a high spatial resolution Digital Surface Model (0.05 m) and the images acquired by the multispectral sensor were used to derive reflectance maps (0.1 m) in the four wavelengths bands. The four reflectance layers were combined to produce two straight-forward vegetation indices (Normalized Difference Vegetation Index and Green NDVI). The photogrammetric DSM’s were combined to a LiDAR Digital Terrain Model (public database, survey in winter 2013) to produce Crop Height Models (CHM) of the study area. We used multiple linear regressions modelling in order to predict the AGB of the field crop monitored by the ICOS station of Lonzée with UAV imagery. AGB=a+b*GNDVI+c*NDVI+d *CHM The field crop data were provided by the ICOS program and by field research conducted in experimental field crops close to the flux tower. The field sampling consisted in destructive samples of the crop which were weighted after drying. For each field sample, an associated area was computed based on the outdistance sowing and the number of sampled crops in order to compute an AGB per area unit (t / Ha). Each AGB field estimation was associated to the closest flight date to build a multi date model presenting good performances (r² = 0.85, RMSE = 2.3 t/Ha). We used the same modelling approach to adjust a single date model to derive a predicted AGB map for the 7th of July. The performance of the single date model is lower but still highlights the biomass variation within the crop (r² = 0.71, RMSE = 1.9 t/Ha). The predicted AGB map displays a high spatial heterogeneity with some spatial patterns. Locally low AGB values are found along two old pedestrian whereas higher AGB values can be associated to areas which were sprayed twice (in-between two tractor tracks). Our results highlight the potential of UAV multispectral imagery to monitor the AGB variation within the footprint of the flux tower and highlight the need for repeated field sampling with a precise geolocation to improve the matching between the flight and the field surveys. [less ▲]

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See detailBeech phenology and productivity at the Vielsalm Terrestrial Observatory
Hurdebise, Quentin ULiege; Vincke, Caroline; De Ligne, Anne ULiege et al

Poster (2017, October 20)

Temperate forests, as other ecosystems and oceans, mitigate the accumulation in the atmosphere of CO2, the main responsible for the current climate change. It is, therefore, a necessity to understand how ... [more ▼]

Temperate forests, as other ecosystems and oceans, mitigate the accumulation in the atmosphere of CO2, the main responsible for the current climate change. It is, therefore, a necessity to understand how these forests react and will react in a changing environment. That requires long-term simultaneous monitoring of the forest productivity and phenology as well as of climatic variables. Using the 20 years dataset from the Vielsalm Terrestrial Observatory (VTO), an eddy covariance site located in a mixed forest in east Belgium and dominated by beeches, the relations between phenological and productivity indicators were analyzed. Phenological indicators (leaf out beginning, duration and ending as well as leaf fall date) were derived from the relative light transmissivity through the canopy and from the temporal dynamic of the Net Ecosystem Productivity (NEP). No trend was observed for these indicators during the last two decades, but significant relations were found between them and between them and climatic variables. Productivity indicators were derived from eddy covariance measurements (NEP), tree ring widths (growth index) and masting intensity. Growth index was significantly related to the NEP and a reverse proportionality to the masting intensity was found. The analysis of the relation between the beech stand NEP and the phenology gives us evidences that annual NEP is significantly related to the length of the carbon uptake period. A model combining the length of the carbon uptake period and the photosynthetic capacity of the ecosystem was able to predict more than two third of the interannual variability at the VTO. This work underlines the multiplicity of the relation between phenological and productivity indicators. [less ▲]

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See detailEnvironmental controls of methanol emissions from a grazed grassland in Dorinne, Belgium
Michel, Colin ULiege; Heinesch, Bernard ULiege; Bachy, Aurélie ULiege et al

Poster (2017, October 20)

Despite the growing interest for oxygenated volatile organic compounds (OVOC) over the last 15 years due to their role in the atmospheric chemistry, current knowledge about OVOC exchanges by grassland and ... [more ▼]

Despite the growing interest for oxygenated volatile organic compounds (OVOC) over the last 15 years due to their role in the atmospheric chemistry, current knowledge about OVOC exchanges by grassland and the environmental factors driving them remains lacunar. However, those ecosystems represent an important part of the total earth surface (13.37%). This study conducted on a grazed grassland therefore aims to quantify OVOC exchanges over full grazing seasons in order to understand the mechanisms behind these exchanges. It took place within the activities of the CROSTVOC project (CROp Stress VOC) and therefore gives an important attention to the stress induced fluxes. BVOC flux measurements were performed with a PTR-MS for the measurements of OVOC mixing ratios on two different scales: the eddy Covariance method was used during two whole grazing seasons (2014 and 2015) and in the 2016 summer, measurements were also performed on a smaller scale by using all-teflon automated dynamic chambers. The chambers allowed measuring accurately the impact of grazing by following simultaneously undisturbed and grazed grassland patches. This study pointed out that several OVOC were exchanged in variable quantities, with methanol being by far the most important. Methanol fluxes exhibited a clear diurnal cycle with close-to-zero fluxes at night and maximum fluxes at midday. The flux was also much larger in the summer than during autumn or spring. For the eddy Covariance data, the average methanol flux in the summer (0.033 μg.m-2s-1) was in the same range of other studies, being lower than the average found by Bamberger et al. in 2010 (0.080 μg.m-2s-1) and by Ammann (0.077 μg.m-2s-1). Fluxes from the chambers were slightly lower with an average value of 0.27 μg.m-2s-1 but they were measured in the late summer. Driver analysis is still ongoing but first results showed that the flux was strongly correlated at short time scale (half hourly for the EC method and one and a half hourly for the chambers) to the photosynthetic photon flux density and the latent heat flux. We believe that their influence comes mainly from the control practiced by stomatal conductance in non-steady state conditions and expansion of cell walls in steady state conditions. The cuvette data showed that grazing resulted in significantly enhanced emissions of methanol that lasted for several days. On the contrary, the eddy Covariance method cannot distinguish between grazed and non-grazed grass and only a small fraction of the method footprint had been grazed the previous days on average. Our eddy-covariance data suggested that an increase in the stocking density did not contribute to a significant rise of methanol emission. The limited variability in stocking density during the two growing seasons and the more powerful effect of other drivers likely induced that the flux variability due to the SD was too low to be measured by the eddy Covariance method. In depth comparisons between the eddy-covariance data and the dynamic chambers data will be carried out. [less ▲]

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See detailPhosphorous from Sludge: Extraction Expertise at ULiège
Pfennig, Andreas ULiege; Shariff, Zaheer Ahmed ULiege

Poster (2017, October 18)

In the PhosForYou project, the PASCH process, formerly designed to recover phosphorous from sewage-sludge incineration ash, is extended to start from dried sewage sludge directly. One of the major steps ... [more ▼]

In the PhosForYou project, the PASCH process, formerly designed to recover phosphorous from sewage-sludge incineration ash, is extended to start from dried sewage sludge directly. One of the major steps in this process is the reactive extraction of heavy metals from a leach liquor, followed by fractional precipitation. The process is realized on pilot-plant scale and tested in bypass at waste-water treatment plants. The process param-eters will be optimized to maximize process efficiency depending on the local situation with respect to impurities and water content. The product is a phosphate, which can directly be used in fertilizer formulations. [less ▲]

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See detailQuality assessment of marketed eggs in Hanoi (Vietnam)
Moula, Nassim ULiege; Luc, Do Duc; Bo, H. X. et al

Poster (2017, October 13)

Poultry farming is the first sector of livestock that has been industrialized. The poultry sector plays an important role in the GDP of Vietnam. It is the second largest livestock after the pig livestock ... [more ▼]

Poultry farming is the first sector of livestock that has been industrialized. The poultry sector plays an important role in the GDP of Vietnam. It is the second largest livestock after the pig livestock. The production and consumption of eggs in Vietnam is estimated as 5.64 billion and 102.6 eggs per person per year respectively. The aim of this study was to assess the quality of eggs in relation to the chicken breed and different marketing channels in Hanoi, Vietnam. In total, 431 chicken eggs were purchased from four different marketing channels in Hanoi, including households (89 eggs), supermarkets (152 eggs), public markets (130 eggs) and small grocery stores (50 eggs). Out of the 431 above-mentioned eggs, 119 eggs came from the commercial chicken breeds and 312 eggs from traditional local breeds. The quality of eggs for a consumer is represented by its cleanliness, nutritional quality, freshness, and price. A series of measurements is carried out just after their purchase. According to the breed and the marketing channel, highly significant differences (P <0.05) were found in the freshness of the eggs (Haugh units), egg prices, eggs weight, egg shell, the white and the yolk of the egg. Though the chicken breed and marketing channels do not significantly affect (P> 0.05) the freshness of the eggs, however, they have a significant effect (P <0.05) on the size of the eggs marketed in Hanoi. The results of this study show an important diversity in marketing channels and marketed eggs in Hanoi. [less ▲]

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See detailInventory of Beekeeping in the Algerian north (Tizi-ouzou and Bejaia)
Yahi, Krimou; Touazi, Leghel; Kaidi, Rachid et al

Poster (2017, October 13)

The objective of this study is to make an inventory of the honey production in the counties of Bejaia and Tizi-Ouzou in northeastern Algeria. To achieve this, a survey was carried out on 31 beekeepers (14 ... [more ▼]

The objective of this study is to make an inventory of the honey production in the counties of Bejaia and Tizi-Ouzou in northeastern Algeria. To achieve this, a survey was carried out on 31 beekeepers (14 in Bejaia and 17 in Tizi-Ouzou). The survey shows a social, cultural and religious role for 61.29% of the beekeepers. The beekeeping is primarily held by men (93.55%). It is the basic source of income for 64.52% of the respondents. The average age of the beekeepers is 42.90years (Min-Max: 28-67 years, Median: 40.5 years). The main products of the hive are honey (100%), swarms (16.13%), royal jelly (9.68%) and propolis (9.68%). The average honey production per hive is 7.70kg (Min-Max: 0.4-15kg hives, Median: 10kg). The average number of hives per beekeeper is 42.20 hives (Min-Max: 3-300 hives, Median: 17.50 hives). The average selling price of one kg of honey is 4000.00 DA / kg (Min-Max: 2000-5000DA; Median: 4000DA). The phenotype of the bee reported by 74.19% of the surveys is of small size with a long body and dark pigmentation corresponding to the breed “Apis mellifera intermissa”. Two apiculturists described another phenotype corresponding to the “Apis mellifera major”. The factors behind the motivations for beekeeping are consumption of honey (100%), income generation (90.32%), hobby (58.06%) and conservation of biodiversity (22.58%). The multiple constraints associated with several diseases, notably Varroase (mentioned by 80.65% of beekeepers), cause difficulties for the breeders. Thus they cannot profit maximum from beekeeping. Other constraints which were reported are; forest fires (35.48%), wasps (32.26%), absence of beekeeping professionals or technicians (29.03%), harsh and cold winters with snow (19.35%), high density of hives in the region (16.13%) and uncontrolled spreading of pesticides and crop protection products at farms (12.90%). The economic situation of the Algerian beekeepers can be optimized by improving the production potential of the local bees. [less ▲]

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See detailComparison of the effects of Opuntia ficus-indica powder on growth performance and serum parameters of the Broiler Chicken in Algeria
Moula, Nassim ULiege; Humbel, Maïlis ULiege; Leterrier, Mélanie ULiege et al

Poster (2017, October 13)

In Algeria, the ingredients used in the broiler feed are exclusively imported from abroad, which affects negatively the production cost of the chicken meat and its sale price in the market at national ... [more ▼]

In Algeria, the ingredients used in the broiler feed are exclusively imported from abroad, which affects negatively the production cost of the chicken meat and its sale price in the market at national level. Because of the wide diversity in soil and climate, Algeria has a substantial number of plants which can be used in animal feed. This work is part of the valuation of the feed potential of the barbaric fig tree, widely present in the Algerian rural landscape, for the broiler chicken. The Opuntia ficus-indica is known for its edible fruits and for use of its fleshy leaves or “racquets” as fodder especially during periods of drought. It is also used to control the water and wind erosion as well as for the protection and improvement of soil fertility in arid and semi-arid regions. The aim of this study was to measure the effects of Opuntia ficus-indica powder on growth performance, serum composition and carcass yield of broiler chickens. The experiments were performed in a private poultry farm in the Chemini region (Wilaya de Bejaia). In this study, 120 Ross-308 day-old male chicks were monitored. They were divided in 3 groups (Group 1, 2 and 3) according to the specific diet (4 x 10 chicks / group). The group 1 was offered commercial feed. The group 2 and group 3 were offered the same commercial feed as group 1 but 5% and 10% of the commercial feed was replaced by the Opuntia ficus-indica powder respectively. The Opuntia ficus-indica powder did not show any negative effect (p>0.05) on the final body-weight, average daily gain and carcass yield. However, it decreased (p<0.05) the biochemical parameters -blood concentration- (plasma glucose, uremia, cholesterol and triglycerides). In conclusion, the Opuntia ficus-indica powder has the potential to be used in poultry feed to reduce the cost of broiler feed in Algeria [less ▲]

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See detailClinical and haematological alterations in foot and mouth disease virus naturally-infected domestic water buffaloes in Vietnam
Dam Van Phai, ULiege; BUI TRAN ANH, Dao, ; Desmecht, Daniel ULiege

Poster (2017, October 13)

Foot and mouth disease (FMD) has been one of the most important epizootic diseases in several cloven-hoofed animals including buffaloes, causing severe economic losses in Vietnam. This study aimed at ... [more ▼]

Foot and mouth disease (FMD) has been one of the most important epizootic diseases in several cloven-hoofed animals including buffaloes, causing severe economic losses in Vietnam. This study aimed at observing clinical indicators, physiological parameters, haematological and blood chemistry values of 30 buffaloes naturally infected with FMD virus (FMDV). The study was undertaken from January 2015 to February 2016 in Hanoi, Vietnam. Results show that buffaloes infected with FMDV displayed significantly higher fever, increased respiratory rate and increased heart frequency compared to healthy animals. Moreover, FMDV-infected animals always showed high fever and vesicular stomatitis, often showed edging nail congestive inflammation and, sometimes only, blisters on the nipple. Haematological indicators showed that FMDV-infected buffaloes were dehydrated (increased total protein). Besides, infected animals also underwent anaemia as mean corpuscular haemoglobin and mean corpuscular haemoglobin concentrations were plummeted. Total white blood cell count plummeted too, especially the number and proportion of neutrophils. Meanwhile, the number and proportion of lymphocytes sharply increased compared to healthy animals. Infected buffaloes also displayed signs of liver and heart damages as judged by an increase in concentration of AST and ALT, while there was not renal damage since creatinine concentration remained stable [less ▲]

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See detailElectrode materials for Li/Na-ion batteries: Improving electrochemical performance through carbon addition during synthesis
Mahmoud, Abdelfattah ULiege; Karegeya, Claude; Eshraghi, Nicolas ULiege et al

Poster (2017, October 12)

Lithium-ion batteries have been widely applied as a power source for portable and stationary energy storage systems. Na-ion batteries are considered to be an alternative to Li-ion batteries owing to the ... [more ▼]

Lithium-ion batteries have been widely applied as a power source for portable and stationary energy storage systems. Na-ion batteries are considered to be an alternative to Li-ion batteries owing to the natural abundance of sodium. New electrode materials are required to increase the energy density of Li/Na-ion batteries. In this study, we show that the addition of the carbon sources during the synthesis leads to control the particles size and morphology and improve their conductivity properties that enhance the electrochemical performance [1-5]. In order to study the effect of the carbon on the structural, morphological and electrochemical properties of the prepared materials by a spray-drying [1-3] or hydrothermal methods [4, 5]. The crystal and local structure were analyzed by XRD and Mössbauer spectroscopy. The morphological properties were characterized by SEM and TEM. The carbon content was determined by TG/TDA and carbon analyzer. The electrochemical properties were studied by impedance spectroscopy and galvanostatic cycling in lithium cells. Finally, the reaction mechanism during cycling was investigated using operando XRD technique. 1- A. Mahmoud, S. Caes, M. Brisbois, R.P. Hermann, L. Berardo, A. Schrijnemakers, C. Malherbe, G. Eppe, R. Cloots, B. Vertruyen, F. Boschini, Spray-drying as a tool to disperse conductive carbon inside Na2FePO4F particles by addition of carbon black or carbon nanotubes to the precursor solution, J. Solid State Electrochem. (2017) 1–10. 2- N. Eshraghi, S. Caes, A. Mahmoud, R. Cloots, B. Vertruyen, F. Boschini, Sodium vanadium (III) fluorophosphate/carbon nanotubes composite (NVPF/CNT) prepared by spray-drying: good electrochemical performance thanks to well-dispersed CNT network within NVPF particles, Electrochim. Acta, 228 (2017) 319–324. 3- M. Brisbois, S. Caes, M-T. Sougrati, B. Vertruyen, A. Schrijnemakers, R. Cloots, N. Eshraghi, R-P. Hermann, A. Mahmoud, F. Boschini, Na2FePO4 F/multi-walled carbon nanotubes for lithium-ion batteries: Operando Mössbauer study of spray-dried composites, Solar Energy Materials & Solar Cells 148 (2016) 67-72. 4- C. Karegeya, A. Mahmoud, B. Vertruyen, F. Hatert, R.P. Hermann, R. Cloots, F. Boschini, One-step hydrothermal synthesis and electrochemical performance of sodium-manganese-iron phosphate as cathode material for Li-ion batteries, J. Solid State Chem.253 (2017) 389–397. 5- C. Karegeya, A. Mahmoud, R. Cloots, B. Vertruyen, F. Boschini, Hydrothermal synthesis in presence of carbon black: Particle-size reduction of iron hydroxyl phosphate hydrate for Li-ion battery, Electrochimica Acta. Electrochim. Acta 250 (2017) 49–58. [less ▲]

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See detailIntramolecular continuous-flow strategy towards methylphenidate (Ritalin) hydrochloride
Gerardy, Romaric ULiege; Winter, Marc; Vizza, Alessandra et al

Poster (2017, October 12)

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See detailSedimentary infill at the western tip of the Gulf of Corinth during the last 120 ka: Evidence for an acceleration of the subsidence
Beckers, Arnaud; Hubert, Aurelia ULiege; Beck, Christian et al

Poster (2017, October 12)

The Corinth Rift, Greece, is a young and active continental rift stretching over 150 km between the cities of Patras and Athens, and is partly covered by the sea forming the Gulf of Corinth. The present ... [more ▼]

The Corinth Rift, Greece, is a young and active continental rift stretching over 150 km between the cities of Patras and Athens, and is partly covered by the sea forming the Gulf of Corinth. The present study is focused on the western tip of the Gulf, west of the town of Aigion, where the extension rate measured by GPS is the highest, reaching 14-16 mm/yr. The sediments were investigated using seismic reflection profiling (600 km) to characterize the evolution over the last 120 ka of the sedimentation, subsidence and faulting activity. We combined two lines of evidence, the position of lowstand deltas and isopach maps. The isopach maps were built using two stratigraphic markers could be traced through the seismic grid, the most recent one corresponds to the last post-glacial transgression and the antecedent one to MIS 6 / MIS 5 transgression, at ca. 130 ka. The related isopach maps evidence a spatial change in sedimentary infill along the rift axis probably related to a decrease in activity of the south-dipping faults (i.e. Trizonia/Mornos Faults) that formed the northern edge of the westernmost Corinth Rift in an early stage of the rifting. The different identified lowstand fluvio-deltaic deposits are related to global sea-level lowstands during which the Gulf of Corinth was a lake, whose last reconnection to the Sea occurred around 11.5 ka. Concerning lowstand deltas formed around 11.5 ka, the subsidence rates exceed 3 mm/yr and are maximal under the apex of the Mornos fan-delta (5.0-6.6 mm/yr) and in the hanging wall of the north-dipping Lambiri fault (5.9-7.5 mm/yr). Regarding the anterior lowstand delta, the subsidence was lower ranging from 1 to 2.7 mm/yr. These changes would arise because of the northward migration of the strain toward the north, e.g. the Marathias fault. [less ▲]

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See detailRelationship between brain activity and ocular movements during wakefulness and drowsiness
François, Clémentine; Wertz, Jérôme; Verly, Jacques ULiege

Poster (2017, October 11)

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See detailA 3000 yr paleoseismological history of the central East Anatolian Fault (Turkey) based on sedimentary record of Hazar Lake
Lamair, Laura ULiege; Hubert, Aurelia ULiege; Hage, Sophie et al

Poster (2017, October 10)

The East Anatolian Fault (EAF) is a major left-lateral strike-slip fault accommodating with the conjugate North Anatolian Fault the westward extrusion of the Anatolian Plate away from the Arabia-Eurasia ... [more ▼]

The East Anatolian Fault (EAF) is a major left-lateral strike-slip fault accommodating with the conjugate North Anatolian Fault the westward extrusion of the Anatolian Plate away from the Arabia-Eurasia collision zone. During the 20th century, the EAF activity was mostly quiestcent with only two events of magnitude greater than 6 recorded (1905 Malatya and the 1971 Bingol earthquakes). Historical seismicity suggests that the EAF is capable of generating earthquakes of magnitude greater than 7. In order to retrace the seismic history of the EAF in its central part, we study Hazar Lake. Hazar Lake is a 20 km long pull-apart basin with a maximum depth of 216 m. Short cores and long sediment cores were collected at four different sites to retrieve a paleoseismological record. Detailed analysis of the sediment cores (e.g. magnetic susceptibility, XRF, XRD, thin sections) were performed to identify sedimentary events. The ages of the sedimentary events were inferred based on a detailed age-depth model combining radiocarbon dating and 137Cs/210Pb. In total, 65 radiocarbon dating were done on bulk sediment and on terrestrial organic matter. The results show that Hazar Lake region was impacted by two fault zones: The East Anatolian Fault (EAF) and the North Anatolian Fault. Based on historical documents, the seismic intensity of each seismic event recorded in Hazar Lake was calculated. Here, we discuss the seismic threshold for earthquake records as well as the seismic recurrence pattern for the EAF over the last 3000 years. [less ▲]

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See detailPhysico-chemical Approach for Characterising Probiotics: example of High Concentrated Multistrain-Based Formulation
Razafindralambo, Hary ULiege; Delvigne, Frank ULiege; Blecker, Christophe ULiege

Poster (2017, October 09)

Probiotics are receiving today unprecedented growing interests in Europe, Asia, and in the rest of the world. Their applications are not only limited to food and health sectors, but cover also agriculture ... [more ▼]

Probiotics are receiving today unprecedented growing interests in Europe, Asia, and in the rest of the world. Their applications are not only limited to food and health sectors, but cover also agriculture and aquaculture areas. The quality of probiotic products depends on many factors such as, the properties of each individual strain and its proportion in mixed products, the viable probiotic dose, and other selective ingredients like prebiotics and protectant agents incorporated into the formulation. Several strategies are employed for ensuring high product qualities, which can be controlled by different methods and techniques. To date, the physico-chemical approach for characterizing and controlling probiotic qualities and performances appears very attractive, but less exploited. It particularly consists in characterizing probiotic products in terms of thermal, surface and colloidal properties, which could be correlated to probiotic viability and functionalities. In this communication, we report the efficiency of such an approach when applied to a high concentrated multistrain-based formulation which has been shown a metabolic variability impacting on the inflammatory response, depending on the production site. [less ▲]

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See detailThe LNQ25 and ELN PVT Metrics Exhibit a Good Sensitivity to Sleep Deprivation and are Independent from the Subject
Latour, Philippe ULiege; Van Droogenbroeck, Marc ULiege

Poster (2017, October 09)

Introduction Performance of people undergoing critical tasks (like driving) may be impaired completely by the lowering of their vigilance level, due to sleep deprivation for instance. This reduction of ... [more ▼]

Introduction Performance of people undergoing critical tasks (like driving) may be impaired completely by the lowering of their vigilance level, due to sleep deprivation for instance. This reduction of performance may be measured by metrics computed from the reaction times (RT) recorded during a 10min Psychomotor Vigilance Test (PVT). Here, we analyze and compare the sensitivity to sleep deprivation and the subject dependent variability of the PVT metrics performance, with a special emphasis on the time interval sizes. Materials and Methods Individuals (22 volunteers; 11 males, 11 females, mean 22.2y., range 19-34 years) follow an uninterrupted 28h sleep deprivation standard PVT protocol during which they achieved two groups of three PVT sessions (in different conditions). The first PVT of each group is in Non-SDP condition (9h30 and 10h30 Day 1) and the second and third PVT of each group are in SDP condition (2h30, 3h30, 10h30 and 11h30 day 2). The subjects fill a sleep journal during the week before the experiment. We checked that they had a normal sleep-wake cycle with no sleep deprivation, jet-lag or shift work and no medication. During the PVT of the first group, the subjects were wearing the glasses of the Phasya’s Drowsimeter. We compute the usual PVT metrics; meanRT, meanRS (Reaction Speed) and LN500 (500ms lapses number). We also compute LNQ25 (adaptive lapses number computed with a subject dependent threshold) and ELN (Expected Lapse Number, computed from a subject-dependent estimation of the lapse probability). Results We use the “Effect Size” (ES, ratio of the mean by the standard deviation of the difference of metrics in the SDP and Non-SDP conditions) to assess the sensitivity to sleep deprivation. For the 10min (resp. 1min, 3min) interval, the ES of LNQ25 and ELN are respectively 1.38 (resp. 0.95, 1.22) and 1.35 (resp. 0.85, 1.14), the ES of meanRS, meanRT and LN500 are 1.23 (resp. 0.91, 1.09), 0.81 (resp. 0.54, 0.68) and 0.85 (resp. 0.63, 0.77). We classify the intervals on which metrics are computed as SDP or non-SDP. We use a fixed threshold for the metrics, independent of the subject. In the ROC curves, the TPR (for a FPR of 10%) assesses the quality of the classification, and then also the subject independence. For the 10min (resp. 1min, 3min) interval, the TPR of LNQ25 and ELN are respectively 0.86 (resp. 0.56, 0.75) and 0.83 (resp. 0.58, 0.75), the TPR of meanRS, meanRT and LN500 are 0.42 (resp. 0.38, 0.41), 0.40 (resp. 0.39, 0.40) and 0.42 (resp. 0.24, 0.30). Conclusions We demonstrate that LNQ25 and ELN enable a quite good classification of the SDP condition for time intervals greater than or equal to 3min, independently of the subject. On the other hand, these metrics provide also a good sensitivity to sleep deprivation. They outperform the usual metrics for both criteria. For time intervals below 3min, the performances degrade first progressively and then much more quickly below 1min. [less ▲]

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See detailRuminal function influenced by diet parameters in dairy herds with milk fat drop syndrome in Belgium
Knapp, Emilie ULiege; Sartelet, Arnaud ULiege; Malniece, Aija et al

Poster (2017, October 06)

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See detailAbdominal steatonecrosis in a twelve-year old Highland bull.
Casalta, Hélène ULiege; Bayrou, Calixte ULiege; Denis, Marine ULiege et al

Poster (2017, October 05)

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See detailAtypical polypoid leiomyosarcoma in an ouessant ewe.
Bayrou, Calixte ULiege; Casalta, Hélène ULiege; Cassart, Dominique ULiege et al

Poster (2017, October 05)

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See detailQuantitative profiling of endogenous polar metabolites from low volumes of blood samples
Kok, Miranda ULiege; Fillet, Marianne ULiege

Poster (2017, October 05)

The etiology of many diseases is not yet completely understood. The involved biological processes might be resolved using a metabolomics approach, because metabolomics provides unique challenging ... [more ▼]

The etiology of many diseases is not yet completely understood. The involved biological processes might be resolved using a metabolomics approach, because metabolomics provides unique challenging opportunities to correlate the metabolome with a physiological or pathophysiological status and provides a vision on the relationships between genes, gene expression, environment and lifestyle. Here, we present the development of two ultra-high performance liquid chromatography (UHPLC) methods coupled to mass spectrometry (MS) for the separation and quantitation of polar metabolites in blood samples. A reversed-phase UHPLC-MS/MS method has been developed to quantify anionic energetic metabolites, whereas hydrophilic interaction chromatography (HILIC)-MS/MS has been used to determine amino acids. Two sample pretreatment procedures have been developed for an optimal recovery of the respective metabolites from whole blood samples. One method involved the precipitation of proteins with acetonitrile and acids. In addition, volumetric absorptive microsampling has been used for the sample preparation. Small and accurate quantities of biological fluids (10 or 20 µL) can be collected with this sampling technique, which is of great interest for volume-limited samples or serial collection of samples. The developed methods have been validated and will be applied to determine differences in metabolite concentrations between plasma samples from patients and controls. This can lead to a breakthrough in the understanding of diseases and can open new therapeutic perspectives. [less ▲]

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See detailOntogenetic and phylogenetic simplification during white stripe evolution in anemonefish
Salis, Pauline; Roux, Natacha; Soulat, Olivier et al

Poster (2017, October 04)

Coral reef fishes provide classical examples of complex colour patterns exhibiting a huge diversity. Most of these species display spots, stripes, repeated lines, eyespots, grids, etc. This diversity in ... [more ▼]

Coral reef fishes provide classical examples of complex colour patterns exhibiting a huge diversity. Most of these species display spots, stripes, repeated lines, eyespots, grids, etc. This diversity in colour patterns might serve for species recognition, camouflage, mimicry and/or warning. To date, work have mainly been focused on the link between colour patterns, ecology and behavior, that is the ultimate role of these patterns. However, the underlying development and cellular mechanisms controlling these patterns and their evolution, that is their proximal mechanisms, are still largely unknown. To address this question, we are using a well-known coral-reef fish models, anemonefishes (Amphiprion and the monotypic Premnas). This tribe (Amphiprionini) within the Pomacentridae is composed of 30 species that display a relatively simple colour pattern made of 0-3 white stripes that are well visible on a yellow to red, brown or even black body background. This simple colour pattern offers a unique opportunity to better delineate the pattern and processes allowing the diversification of such diversity. Here, we focus on the vertical white stripes present in most species of Amphiprion. We first map their striped patterns on the anemonefish evolutionary tree and reconstruct the ancestral state. Our results provide evidences that the diversification in colour pattern in anemonefish results from successive losses of stripes during evolution. Then, by an ontogenetic study, we show that larvae stripes always appear from rostral to caudal. Interestingly, larvae of some species such as A. frenatus have surplus stripes (with a maximum of three stripes) which disappear caudo-rostrally during the juvenile phase to acquire their adult color pattern. The reduction of stripes number over ontogeny totally matches the sequences of stripe losses across evolution. This demonstrates that the diversification in colour pattern among anemonefish lineages results from changes in developmental processes. Finally, assuming that the number of stripes may be related to the species ecology, we further determined the links between the number of stripes and ecomorphological traits. Together, this innovative study allows to understand how developmental processes are shaping the diversification of color pattern of anemonefishes and how it may be related with ecomorphological traits evolution. [less ▲]

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See detailValidité et utilité d'un test d'orientation dans le secteur de la santé en Fédération Wallonie Bruxelles
Detroz, Pascal ULiege; Monseur, Christian ULiege; Romainville, Marc et al

Poster (2017, October 04)

La Fédération Wallonie Bruxelles de Belgique n’avait pas, jusqu’en 2016, de Filtres à l’entrée des études de Médecine. Elle avait par contre un test diagnostique, formatif, obligatoire, mais non ... [more ▼]

La Fédération Wallonie Bruxelles de Belgique n’avait pas, jusqu’en 2016, de Filtres à l’entrée des études de Médecine. Elle avait par contre un test diagnostique, formatif, obligatoire, mais non contraignant (il n'avait pas d'effet sur l'avenir des étudiants). Ce test se présentait sous la forme d’un questionnaire à choix multiple (QCM) évaluant plusieurs matières : la chimie, la physique, la biologie, les mathématiques, le français et l’anglais. Il se déroulait en même temps sur le site des cinq universités concernées par les études dans le domaine de la santé de la FWB. Les questions du test sont identiques dans ces différentes universités. Il a été organisé entre 2013 et 2016 , deux fois par année en juillet et septembre. L’enjeu était de permettre aux étudiants de mesurer leur compétence à l’entrée des études pour se rassurer (pour les plus fort d’entre eux), pour s’améliorer à travers des activités de remédiation, ou éventuellement pour renoncer (pour les pl us faible d’entre eux). La présente étude a pour objet d’évaluer l’efficacité de ce test diagnostic. Concrètement, les objectifs de l'étude décrite étaient de/d’ : 1. Analyser la qualité psychométrique du test d'orientation. 2. Établir le lien entre les données biographiques de l’étudiant et les résultats au test d'orientation. 4. Établir le lien entre les résultats au test d'orientation et l’inscription réelle en faculté de médecine.(l'effet de dissuasion du test) 3. Établir le lien entre le test d'orientation et la réussite globale et par matière à la fin de la première année. Nos conclusions générales sont les suivantes : Les tests d’orientation présentent une bonne qualité psychométrique. C’est toutefois plus vrai pour les disciplines scientifiques que pour l’anglais et le français. Le test vise bien les aptitudes spécifiques et les compétences prérequises pour entreprendre des études supérieures dans le secteur de la santé. Les candidats proviennent d’un milieu socio-économique élevé et présentent un parcours scolaire au dessus de la moyenne. Ces variables expliquent, chez les étudiants diplômés du secondaire en Belgique, plus de 30% de la variance du score de sciences au test d'orientation (score factorisé à partir des scores de maths, bio, chimie et physique) et plus de 10% du score de langue (score factorisé à partir des scores de français et d’anglais) à ce même test. Ces mêmes variables de présage expliquent aussi en partie le résultat en fin de premier bachelier (22% de variance expliquée pour les étudiants diplômés en Belgique, 34% pour les étudiants diplômés en France). Les résultats aux tests d’orientation, quant à eux, expliquent ensemble 29% de la variance aux résultats de bac1 pour les étudiants diplômés en Belgique et 17% pour ceux diplômés en France. Le test de sciences s’avère beaucoup plus prédictif que le test de langues Les test dissuade peu les étudiants les plus faibles de s’inscrire. Ces résultats seront présentés lors de la communication [less ▲]

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See detailDevelopment of real-time PCR targets for the detection of Tenebrio molitor and Hermetia illucens
Marien, Aline; Debode, Frédéric; Aerts, Céline et al

Poster (2017, October 01)

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See detailNew low electron flux facility in the 0 to 3.5 MeV range for the study of induced signal in JUICE instruments: UVS and MAJIS measurements
Carapelle, Alain ULiege; Grodent, Denis ULiege; Langevin, Yves et al

Poster (2017, October)

We designed and built a new test facility to investigate signal induced by electrons in the 0-3.5 MeV in the JUICE UVS and MAJIS instruments. The facility uses radioisotopes sources to produce low flux of ... [more ▼]

We designed and built a new test facility to investigate signal induced by electrons in the 0-3.5 MeV in the JUICE UVS and MAJIS instruments. The facility uses radioisotopes sources to produce low flux of electrons (< 6000 electrons/cm².s). We present the facility, its capabilities and the results of measurements on UVS and MAJIS. [less ▲]

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See detailPrevalence and characteristics of group B streptococcus colonization in HIV-infected pregnant women in Belgium
DAUBY, Nicolas; ADLER, Catherine; Y MIENDJE DEYI, Véronique et al

Poster (2017, October)

Objectives: High incidence of GBS sepsis has been reported in HIV-exposed but uninfected (HEU) infants in both developed and developing countries, particularly late-onset diseases. We aimed determining ... [more ▼]

Objectives: High incidence of GBS sepsis has been reported in HIV-exposed but uninfected (HEU) infants in both developed and developing countries, particularly late-onset diseases. We aimed determining the prevalence, the characteristics and the risk factors of GBS carriage in HIV-infected and HIV uninfected pregnant women (PW). Methods : Between 1/01/2011 and 31/12/2013, HIV-infected (n=132) and uninfected (n=123) PW had recto-vaginal swabs for GBS detection performed at 35-37 weeks of gestation and at deliver Demographic, obstetrical and medical data related to HIV-infection were prospectively collected. Serotyping of GBS strains was performed on a limited number of randomly selected samples (26 from HIV-infected and 13 from uninfected PW). Results : The overall prevalence of GBS carriage was not statistically different between HIV-infected and uninfected PW (31% vs 24,4% respectively). Age, nadir CD4 cell count, CD4 cell count at delivery and detectable viral load at delivery were not associated with GBS carriage rate in HIV-infected PW. A distinct pattern of GBS serotype was found in HIV-infected PW who were predomina colonized by serotype III (12/26) while HIV-uninfected PW were mostly colonized by serotype Ia (8/13) (p<0,05). Conclusions: As previously reported in other countries, HIV-infected PW do not have significantly higher rate of GBS colonization. However, our results suggest that HIV-infected PW are more lik be colonized with serotype III strains, that is the main serotype associated with late-onset neonatal sepsis. Ongoing research aims at characterizing the clonal features of the isolated strains [less ▲]

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See detailLifetime of 5 non-commercial boluses evaluated in dairy cows by X Rays and plasmatic trace elements concentrations
Guyot, Hugues ULiege; Cheleux, Gaël ULiege; Wajda-Dubos, Jean-Pierre et al

Poster (2017, October)

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See detailPrevalence and capsular-polysaccharide type distribution of colonizing group B streptococci (GBS) isolated from recto-vaginal samples in pregnant women in Hanoï, Vietnam
MEEX, Cécile ULiege; DEVEY, Anaïs ULiege; PHAM HONG, Nhung et al

Poster (2017, October)

Background: The study was organized by the Belgian National Reference Center (NRC) for Streptococcus agalactiae or GBS, and carried out in Vietnam. The aim of the study was to determine the prevalence of ... [more ▼]

Background: The study was organized by the Belgian National Reference Center (NRC) for Streptococcus agalactiae or GBS, and carried out in Vietnam. The aim of the study was to determine the prevalence of GBS colonization among pregnant women in Hanoï and to characterize the capsular-polysaccharide (CPS) type of the isolated strains. Methods: For a 2-months period in 2015, 888 recto-vaginal swabs were collected in Bach-Mai-Hospital from pregnant women at 35-37 weeks’ gestation and were cultured for detection of GBS. Strains were stored and transferred to the Belgian NRC for further characterization. CPS-typing was performed by both latex agglutination and PCR (Poyart, 2007; Kong, 2008). Results: Among the 888 swabs, 111 were positive for GBS, that is a prevalence of colonization of 12.5%. A total of 90 strains were available for typing: 91,11% could be serotyped by latex agglutination and all the strains, including the 8 phenotypically non-typable strains, were successfully genotyped. CPS type V was the most prevalent (36.7%) followed by CPS types Ib (25.6%), III (21.1%), VI and VII (8.9% and 4.4%). CPS type II was found twice and serotype Ia was found once. CPS types IV, VIII and IX weren’t present in this population. Conclusion: With predominance of types V, Ib and III, this distribution of CPS-types of GBS colonizing pregnant women in Hanoï, Vietnam, differs from distributions described in Europe and in o Asian countries. This study provides useful information for the development of a universal vaccine that could contribute to improve the prevention of neonatal GBS infections. [less ▲]

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See detailEvolution of rate and genotypes of resistance to macrolide/lincosamide among invasive Group B Streptococcus (GBS): Development of a multiplex PCR tool for simultaneous detection of ErmB, ErmTr, MefA and LsaC resistance genes.
SACHELI, Rosalie ULiege; DESCY, Julie ULiege; MEEX, Cécile ULiege et al

Poster (2017, October)

Methods: A multiplex-PCR, using a set of specifically designed or already described (Kataja, 1999; Malbruny, 2011) primers was developed and used to detect, in GBS, three genes for erythromyc resistance ... [more ▼]

Methods: A multiplex-PCR, using a set of specifically designed or already described (Kataja, 1999; Malbruny, 2011) primers was developed and used to detect, in GBS, three genes for erythromyc resistance, ermB, ermTR, mefA and one gene for clindamycin-resistance lsaC. AdhP gene amplification was used as control for GBS identification. All(219) GBS isolates from invasive infections in newborns and adults received by the Belgian National Reference Center for GBS in 2015, and control strains were tested for erythromycin/clindamycin susceptibility (disk-diffusion/broth- microdilution) and for detection of resistance genes. Results: PCR products demonstrated the expected respective sizes. The method has been validated successfully according to ISO15189 analytical requirements. Of the 219 isolates, 67(30,67%) w resistant to erythromycin and/or clindamycin: 44/67(65,78%) showed a constitutive-MLS phenotype and 10/67(14,9%) the inducible-MLS phenotype. Among the constitutive-MLS strains, 73% harboured ErmB gene, 13% ErmTR, 7% ErmB+mefA and 7% ermB together with LsaC gene. The inducible-MLS strains harboured mostly ErmTr gene (89%) and the others the ErmB gene. Among the 10/67(14,9%) GBS strains with an M-phenotype (isolated resistance to erythromycin), the MefA gene was exclusively detected. Among the 3(4,48%) strains showing an isolated resistance to clindamycin (L-phenotype), the LsaC gene was detected. Conclusion: The developed multiplex PCR is able to detect simultaneously four genes involved in MLS resistance in GBS. In 2015, 30,6% of the invasive GBS strains isolated in Belgium were resist to macrolides and/or lincosamides. The emergence of the L-phenotype in GBS described since 2010, justifies the relevance to also detect LsaC gene together with ErmB, ErmTr and MefA. [less ▲]

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See detailUpdate of the characteristics of Group B Streptococci (GBS) colonizing pregnant women in Belgium: capsular-type distribution, pili characterization, antimicrobial susceptibility profile and Multiple Locus Sequence Types.
SACHELI, Rosalie ULiege; MEEX, Cécile ULiege; DESCY, Julie ULiege et al

Poster (2017, October)

Aim: Improving knowledge and characterization of GBS strains colonizing pregnant women in Belgium. Methods: In 2013, collection of 387 strains of GBS from 80 laboratories participating in a national ... [more ▼]

Aim: Improving knowledge and characterization of GBS strains colonizing pregnant women in Belgium. Methods: In 2013, collection of 387 strains of GBS from 80 laboratories participating in a national survey among pregnant woman. For each strain, determination of capsular-polysaccharide type agglutination and PCR, of pili-type by PCR and of antimicrobial susceptibility by disk-diffusion, broth-microdilution and detection of resistant genes by PCR. For serotype III strains, determination sequence-type by Multiple-Locus Sequence-Typing (MLST). Results: Serotype III was the most prevalent (28.5%) followed by serotypes V, Ia, II, IV and Ib (20.4%, 19.9%, 17.8%, 7%, 5.4%). Serotypes VI, VII and IX were found each once. All strains remained susceptible to penicillin (MICs: 0.03-0.125 mg/L) and other beta-lactams tested; 28.7% were resistant to erythromycin and 26.7% to clindamycin. With regards to pili, all 387 strains harboured one the PI-2 variants alone or in combination and 70.3% contained PI-1. The 110 serotype III isolates were resolved into 18 STs. The most common were ST-17 (35.5%) followed by ST-19 (30%) and ST- ST-27, ST-23 (<=5%). Conclusion: Among GBS from colonized pregnant women in Belgium: capsular-type and pili distributions, and MLST profile among type III strains were quite similar to reported data from Europ and USA during the last decade. As showed in this study, penicillin remains the first line drug of choice. On the contrary, resistance rates against macrolides/lincosamide, has reached a plateau since a decade, but it is noteworthy to notify the emergence of strains with isolated resistance to clindamycine. [less ▲]

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See detailIntramolecular Continuous-Flow Strategy Towards Methylphenidate (Ritalin) Hydrochloride
Gerardy, Romaric ULiege; Winter, Marc; Vizza, Alessandra et al

Poster (2017, October)

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See detailStratospheric circulation in the WACCM chemistry-climate model: mean age of air against observations and CTM
Minganti, Daniele ULiege

Poster (2017, October)

The mean age of air is a classical diagnostic of the transit time from the troposphere to the various regions of the stratosphere, providing insights on the strength and structure of the Brewer-Dobson ... [more ▼]

The mean age of air is a classical diagnostic of the transit time from the troposphere to the various regions of the stratosphere, providing insights on the strength and structure of the Brewer-Dobson Circulation (BDC), the polar vortex and the irreversible mixing in the mid-latitudes. We apply this diagnostic to the WACCM chemistry-climate model for the 1985-2014 period. A comparison is presented between unconstrained simulations (FR-WACCM) with and without the representation of the QBO, simulations nudged to the MERRA-2 reanalysis (SD-WACCM), in-situ measurements and a chemistry-transport model (CTM). The results are different depending on the simulation, with SD-WACCM resulting closer to the observations w.r.t. FR-WACCM. In the FR-WACCM the QBO plays a role leading to older age of air. The time evolution over the considered period is slightly decreasing, while observations show a positive (not significant) trend. Given those results, further studies have to be carried out investigating the multi-decadal trends of mean age of air and involving actual tracers. [less ▲]

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See detailDrainage vaginal spontané d'un abcès appendiculaire chez une fillette de 7 mois.
BRICHANT, Géraldine ULiege; FRERE, Julie ULiege; El Hachemi, Mounia et al

Poster (2017, October)

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See detailA 5-year survey of dermatophytes strains circulating in Belgium
SACHELI, Rosalie ULiege; DARFOUF, Rajae ULiege; ADJETEY BAHUN, Akolé ULiege et al

Poster (2017, October)

Objectives Dermatophytosis refers to superficial fungal infections of keratinized tissues caused by keratinophilic dermatophytes. They are the most common cause of superficial fungal infections worldwide ... [more ▼]

Objectives Dermatophytosis refers to superficial fungal infections of keratinized tissues caused by keratinophilic dermatophytes. They are the most common cause of superficial fungal infections worldwide. Epidemiological studies regarding dermatophytes infections have been conducted in several countries and differences in the incidence and in etiological agents have been reported. That is why national surveillance of circulating strains causing dermatophytosis is critical. The National Reference Center (NRC) for mycoses conducted a survey on dermatophytes strains circulating in Belgium from 2012 to 2016. The present study was performed to assess the profile of dermatophytosis and to identify the species involved. Methods The Belgian NRC for mycoses (Leuven and Liège) collected 14227 strains between January 2012 and December 2016. The strains were obtained from clinically suspected fungal infections of skin, hair and nails. Strains were collected from Belgian laboratories in order to confirm the fungal identification which was performed by microscopy and in case of doubt by ITS sequencing. Results Among the 14227 samples collected, 6248 were identified as dermatophytes (44%). Trichophyton rubrum was the most prevalent species accounting for 61,3% (n=3820) of the strains collected from all sources, followed by T. mentagrophytes complex (19,2%, n=1199) according to the ancient classification (including T. interdigitale, T. benhamiae and T. mentagrophytes). Other less prevalent species were also recorded: M. audouinii (n=507, 8,1%), M. canis (n=210, 3,3%), T. tonsurans (n= 140, 2,2%), T. violaceum (n=133, 2,1%), T. soudanense (n=125, 2%), M. praecox (n=60, 0,96%) and E. floccosum (n=19, 0,3%) for the main ones. Our data show the predominance of anthropophilic species causing tinea capitis especially M. audouinii responsible for 43,4% (n=303/716) of hair/scalp infection with an increasing number from 2014 to 2016. Trichophyton soudanense, rarely observed in Belgium in the past, is an emerging agent of tinea capitis particularly since 2013, accounting for 11,3% (n=81) of the cases during the 5-year study period. Zoophilic strains such as M. canis which were well represented in the past epidemiology of tinea capitis are decreasing accounting for only 8,8% (n=63) of hair/scalp infection. Finally, our data confirm the high prevalence of T. rubrum as the main etiologic agent of onychomycosis (78,1%, n=3094/3968) followed by T. mentagrophytes complex (18,8%, n=743/3968). Both latter strains were also responsible for the majority of skin infections as they were isolated respectively in 46,2% (n=693/1612) and 21,7% (n=348/1612) of skin samples. Conclusions The present epidemiological survey provides recent data on the prevalence of all dermatophytes circulating in Belgium. Analyzing such data is critical for the establishment of measures for prevention and control of dermatophytes infections. Our study confirms the predominance of T. rubrum followed by species from the ancient T. mentagrophytes complex (T. interdigitale + T. benhamiae) in the Belgian population. This survey highlights also the persistent predominance of M. audouinii and the emergence of T. soudanense as causative agents of tinea capitis. [less ▲]

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See detailComparison of four methods for the identification of Trichophyton benhamiae strains: Maldi-Tof MS, ITS sequencing, Diversilab® system and DermaGenius® Multiplex Real-Time PCR kit.
SACHELI, Rosalie ULiege; Ferrer Clusella, Aida ULiege; ADJETEY BAHUN, Akolé ULiege et al

Poster (2017, October)

Background: Infections due to the zoophilic dermatophyte Trichophyton benhamiae are being more frequently diagnosed in Belgium since a few years. This species has been recently included in the T ... [more ▼]

Background: Infections due to the zoophilic dermatophyte Trichophyton benhamiae are being more frequently diagnosed in Belgium since a few years. This species has been recently included in the T. benhamiae series together with T. concentricum, T. verrucosum and T. erinacei (De Hoog et al 2016). The strains appear in culture with a bright yellow thallus and do not easily sporulate making them difficult to identify by microscopy. Maldi-Tof MS may present an alternative to microscopic or molecular identification of this species. Molecular methods as ITS sequencing, Rep-PCR by Diversilab® system and realtime PCR by DermaGenius® kit, have been evaluated as identification methods. Methods: A total of 37 strains have been analyzed, all collected by the National Reference Center for mycosis between 2012 and 2016. Strains appearing with a yellow pigment in culture were compared to strains appearing white in culture and presenting characteristics of Trichophyton interdigitale by microscopy. All strains were characterized by Maldi-Tof MS (Bruker), ITS sequencing, rep-PCR (Diversilab® system, bioMérieux) and real-time PCR (DermaGenius® Nail multiplex kit, Pathonostics). Results: Among the 37 strains, ITS sequencing identified 10 T. benhamiae and 27 T. interdigitale. Most of the time, white strains gave rise to T. interdigitale identification while strains with yellow pigmentation were identified as T. benhamiae. However, two strains were identified as T. benhamiae but did not produce clear yellow pigmentation. DermaGenius®kit was able to detect the same 27 white strains previously identified by ITS sequencing as T. interdigitale but nor the 8 strains with yellow pigmentation nor the two white strains identified as T. benhamiae by ITS sequencing could be recognized. This is explained by the lack of specific designed primers and probes included in the kit. The Diversilab® system classified 19 white strains in a unique cluster belonging to T. interdigitale. Six strains producing yellow pigment as well as the two white strains identified as T. benhamiae by ITS sequencing belonged to a single cluster sharing only 75% of similarity with the cluster of T. interdigitale strains. Finally Maldi-Tof MS could identified14 white strains as T. interdigitale, one white strain was identified as T. benhamiae and 4 analyzed strains producing a yellow pigment were identified as T. benhamiae (51,5% of correct identification). T. interdigitale strains and T. benhamiae strains formed two different clusters easily visualized on the dendrogram. Conclusion: ITS sequencing is the method of choice to identify T. benhamiae strains. The DermaGenius® kit is not yet able to detect this emerging species. Improvement of this method is necessary as the number of T. benhamiae isolated in Europe is increasing. Rep-PCR by Diversilab® is a typing method able to classify the strains into two different clusters of strains belonging to T. interdigitale and T. benhamiae, sharing few similarities and justifying the change of classification of T. benhamiae outside the ancient T. mentagrophytes complex. Finally, Maldi-Tof MS in our settings could identify only 50% of T. benhamiae strains and improvement of the extraction method is necessary to increase the method efficiency. [less ▲]

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See detailPsychological Predictors and Consequences of Hearing Health Behaviors in Older Adults
Marquet, Manon ULiege; Chasteen, Alison L; Plaks, Jason E et al

Poster (2017, September 22)

Introduction: Hearing loss is a common chronic condition affecting older adults in Western populations [1, 2]. Even if older adults could benefit from hearing aid use, they are reluctant to wear them [1 ... [more ▼]

Introduction: Hearing loss is a common chronic condition affecting older adults in Western populations [1, 2]. Even if older adults could benefit from hearing aid use, they are reluctant to wear them [1]. The purpose of the present study was twofold: a) to examine whether older adults’ implicit theories regarding the modifiability of abilities in general would predict their hearing health behaviors; and (b) to assess consequences of these behaviors on subjective aging. Methods: One hundred and sixty-three older adults (Mean age = 68.62, SD = 5.40) completed, among other questionnaires, questions assessing their implicit theories of ability (i.e., seeing general abilities as fixed vs. improvable with effort [3]) and their subjective age (i.e., felt age, do age, look age, and interest age [4]). They also reported their perceptions of the benefits of various hearing health behaviors for slowing the effects of aging (i.e., getting their hearing tested and using hearing aids) as well as their intentions to engage in those behaviors. Results: We found that the more older people considered that abilities are alterable, the more they perceived hearing health behaviors as being beneficial, which in turn increased their intentions to engage in these behaviors. Also, the greater their intentions, the younger subjective age they reported. Key conclusions: These relationships between implicit theories, hearing health behaviors, and subjective age are particularly important to take into account. Indeed, neglecting hearing problems and experiencing an older subjective age may have detrimental health-related outcomes over time [5, 6]. 1. Feder, K., Michaud, D., Ramage-Morin, P., McNamee, J., & Beauregard, Y. (2015). Prevalence of hearing loss among Canadians aged 20 to 79: Audiometric results from the 2012/2013 Canadian Health Measures Survey. Health Reports, 26(7), 18-25. 2. Lin, F. R., Thorpe, R., Gordon-Salant, S., & Ferrucci, L. (2011). Hearing loss prevalence and risk factors among older adults in the United States. The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences, 66(5), 582-590. doi: doi:10.1093/gerona/glr002 3. Dweck, C. S. (1999). Self-theories: Their role in motivation, personality, and development. Philadephia, PA: Psychology Press. 4. Kastenbaum, R., Derbin, V., Sabatini, P., & Artt, S. (1972). “The ages of me”: Toward personal and interpersonal definitions of functional aging. The International Journal of Aging and Human Development, 3(2), 197-211. 5. Bainbridge, K. E., & Wallhagen, M. I. (2014). Hearing loss in an aging American population: Extent, impact, and management. Annual Review of Public Health, 35, 139-152. doi: 10.1146/annurev-publhealth-032013-182510 6. Kotter-Grühn, D., Kornadt, A. E., & Stephan, Y. (2015). Looking beyond chronological age: Current knowledge and future directions in the study of subjective age. Gerontology, 62(1), 86-93. doi: 10.1159/000438671 [less ▲]

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See detailMyoferlin controls mitochondrial structure in pancreatic ductal adenocarcinoma, and affects tumor aggressiveness
Rademaker, Gilles ULiege; Hennequière, Vincent ULiege; Brohée, Laura ULiege et al

Poster (2017, September 22)

Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer, and the third leading cause of cancer related death. Therapeutic options remain very limited and are still based on ... [more ▼]

Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer, and the third leading cause of cancer related death. Therapeutic options remain very limited and are still based on classical chemotherapies. Cell fraction can survive to the chemotherapy and is responsible for tumor relapse. It appears that these cells rely on OXPHOS for survival. Myoferlin, a membrane protein involved in cell fusion was recently shown by our laboratory to be overexpressed in pancreatic cancer. In the present study, we discovered that myoferlin was more expressed in cell lines undergoing oxidative phosphorylation (OXPHOS) than in glycolytic cell lines. In the former cell lines, we showed that myoferlin silencing reduced OXPHOS activity and forced cells to switch to glycolysis. The decrease in OXPHOS activity is associated with mitochondrial network disorganization. Dynamin-related protein (DRP)-1 phosphorylation led us to suggest mitochondrial fission, reducing cell proliferation, ATP production and inducing autophagy and ROS accumulation. To confirm the clinical importance of myoferlin in PDAC, we showed that low myoferlin expression was significantly correlated to high overall survival. Myoferlin staining of PDAC sections was negatively correlated with several 18FDG PET indices indicating that glycolytic lesions had less myoferlin. As the mitochondrial function is demonstrated to enhance the cell resistance to the treatment, the metabolic switch forced by myoferlin silencing could open up a new perspective in the development of therapeutic strategies. [less ▲]

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See detailTowards the development of a dual mode fluorescence/SERS dopamine aptasensor
Dumont, Elodie ULiege; De Bleye, Charlotte ULiege; Cailletaud, Johan ULiege et al

Poster (2017, September 21)

This work was dedicated to the development of a dual mode fluorescence and surface-enhanced Raman scattering (SERS) dopamine aptasensor. The preparation of the aptasensor was first optimised. Thereafter ... [more ▼]

This work was dedicated to the development of a dual mode fluorescence and surface-enhanced Raman scattering (SERS) dopamine aptasensor. The preparation of the aptasensor was first optimised. Thereafter, calibration curves were realised by means of fluorescence quenching and SERS. Finally, the specificity of both methods was tested. [less ▲]

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See detailAgeism and dementia stigma among healthcare professionals
Schroyen, Sarah ULiege; Adam, Stéphane ULiege; Missotten, Pierre ULiege

Poster (2017, September 21)

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See detailEffect of solution treatment on the fracture mechanisms of AlSi10Mg parts produced by Selective Laser Melting
Delahaye, Jocelyn ULiege; Mertens, Anne ULiege; Dedry, Olivier ULiege et al

Poster (2017, September 21)

Al alloy AlSi10Mg processed by Selective Laser Melting (SLM) displays high hardness and tensile strength due to its very fine eutectic microstructure [1]. However, as-built material exhibits low ductility ... [more ▼]

Al alloy AlSi10Mg processed by Selective Laser Melting (SLM) displays high hardness and tensile strength due to its very fine eutectic microstructure [1]. However, as-built material exhibits low ductility which is detrimental for applications where a high resilience is needed. To overcome this issue, solution heat treatment can be applied to soften and ductilize the material [2]. The very fine eutectic microstructure then turns into a dispersion of coarse globular Si precipitates. The distribution of Si precipitates seems to play a role in the fracture mechanism in as-built as well as solutionized tempers, but this effect is not yet fully understood [1,3]. Moreover, the lack of quantitative data makes it difficult to link precisely the fracture mechanisms to specific microstructural features. This study aims at investigating the role of the Si phase in the rupture mechanism, for both as-built and solutionized samples. It will focus more particularly on the heterogeneity of the distribution of Si precipitates where cracks are more likely to occur. Al cells size, along with the volume fraction and distribution of Si precipitates have been quantified by SEM image analysis. Al lattice parameter and Si crystallite size have been determined by X-ray diffraction/Rietveld analysis. For the image analysis, two types of specific zones have been observed i.e. the core and boundary of melt pools and Al grains, at different locations for representativity. These quantitative data have been compared with typical features observed on the fractured surface of tensile specimen. 1. D.-K. Kim et al. J. Alloys Compd. 686 (2016) 281. 2. W. Li et al., Mat. Sci. & Eng. A. 663 (2016) 116, 3. N.T. Aboulkhair et al. Mat. Sci. & Eng. A. 667 (2016) 139. [less ▲]

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See detailNon-classical structures and association of the OXPHOS complexes in Euglena gracilis and Polytomella sp.
Miranda Astudillo, Héctor Vicente ULiege; Yadav, Sathish; Colina-Tenorio, Lilia et al

Poster (2017, September 21)

To date, the idea that the OXPHOS complexes can associate with each other in larger structures named supercomplexes [1] is generally accepted. This association can allow a more efficient transport of ... [more ▼]

To date, the idea that the OXPHOS complexes can associate with each other in larger structures named supercomplexes [1] is generally accepted. This association can allow a more efficient transport of electrons to minimize the generation of reactive oxygen species during electron transfer reactions and can also be involved in the regulation of the mitochondrial metabolism in response to different stimuli, carbon sources or stress conditions. Recently, the arising studies outside the classical models yeast and bovine mitochondria brought to light many unusual characteristics in the ATP synthase from a wide variety of organisms. Due to this, the dimer nature of the mitochondrial ATP synthase is no longer a matter of debate. Given the large structural differences among the peripheral stator and dimerization modules of mitochondrial ATP synthases described so far, it is of relevance to study a wider number of species to gain insight into the structural diversity of their OXPHOS complexes. Recently, our group showed that at least 41 of the non-canonical subunits reported in trypanosomes are also present in Euglena complexes along with 48 classical subunits described in other eukaryotes including green plants [2]. Further purification of the complexes I, III, IV and V by liquid chromatography after solubilization with n-β-dodecyl-maltoside and the subsequent analysis by single-particle analysis from transmission electron microscopy revealed some unusual features in Euglena respiratory complexes. In the case of complex V the structures of both the catalytic and central rotor parts are conserved while other structural features are original, including a large membrane-spanning region joining the monomers, an external peripheral stalk and a structure that goes through the membrane and reaches the inter membrane space below the c-ring [3]. Complex I also shows an unusually long matricial arm. Complex IV shows an atypical shape compared to that of the bovine one. An unusual association between complexes I and V can be observed when the membranes are extracted with the mild detergent digitonin. Other case of atypical subunit composition is the complex V of chlorophycean algae. Each monomer of the enzyme has 17 polypeptides, eight of which are the conserved, main functional components, and nine polypeptides (Asa1 to Asa9) unique to this lineage that form the robust peripheral stalk in this complex [4]. This complex presents highly stable dimeric and tetrameric structures, and the respiratory complexes have an unusual capacity to reassociate in vitro and restore the functional respirasome. [less ▲]

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See detailAtypical composition and structure of the mitochondrial dimeric ATP synthase from Euglena gracilis
Miranda Astudillo, Héctor Vicente ULiege; Yadav, Sathish; Colina-Tenorio, Lilia et al

Poster (2017, September 21)

Mitochondrial respiratory-chain complexes fromEuglenozoa comprise classical subunits described in other eukaryotes (i.e. mammals and fungi) and subunits that are restricted to Euglenozoa (e.g. Euglena ... [more ▼]

Mitochondrial respiratory-chain complexes fromEuglenozoa comprise classical subunits described in other eukaryotes (i.e. mammals and fungi) and subunits that are restricted to Euglenozoa (e.g. Euglena gracilis and Trypanosoma brucei). Herewe studied the mitochondrial F1FO-ATP synthase (or Complex V) fromthe photosynthetic eukaryote E. gracilis in detail. The enzymewas purified by a two-step chromatographic procedure and its subunit compositionwas resolved by a three-dimensional gel electrophoresis (BN/SDS/SDS).Twenty-two different subunits were identified by mass-spectrometry analyses amongwhich the canonical α, β, γ, δ, ε, and OSCP subunits, and at least seven subunits previously found in Trypanosoma. The ADP/ATP carrierwas also associated to the ATP synthase into a dimericATP synthasome. Single-particle analysis by transmission electron microscopy of the dimeric ATP synthase indicated that the structures of both the catalytic and central rotor parts are conserved while other structural features are original. These new features include a large membrane-spanning re- gion joining the monomers, an external peripheral stalk and a structure that goes through the membrane and reaches the inter membrane space below the c-ring, the latter having not been reported for any mitochondrial F-ATPase. [less ▲]

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See detailBreed fast, die young - Demography of a Neotropical fossorial frog
Székely, Diana ULiege; Székely, Paul; Stănescu, Florina et al

Poster (2017, September 19)

Information regarding age- and size-related parameters is of paramount importance both to understanding the life-history of populations, and for designing effective conservation strategies; however, such ... [more ▼]

Information regarding age- and size-related parameters is of paramount importance both to understanding the life-history of populations, and for designing effective conservation strategies; however, such base-line data lacks for many species, especially for those inhabiting tropical habitats. Using skeletochronology, we provide the first detailed information regarding the demography of the Pacific horned frog, Ceratophrys stolzmanni, a vulnerable, fossorial amphibian, that lives in the Tumbesian dry-forests of Ecuador. The species shows an intense growth rate during the first activity season, both before and after metamorphosis. Sexual maturity is achieved by both sexes before the first year of life, after which growth rates abruptly decrease. Females are on average larger, but not older, than males, mean age being two years in both sexes. The rapid sexual maturation is associated with a short life-span, the observed maximum longevity being three years in males and four years in females. The short reproductive life-span, which offers few mating opportunities, might have important implications for the conservation of this species, that can be especially at risk in the case of prolonged droughts expected to affect its habitat. [less ▲]

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See detailChallenges in the determination of amyloid oligomeric species by two electrophoretic techniques
Napp, Aurore ULiege; Houbart, Virginie ULiege; Demelenne, Alice ULiege et al

Poster (2017, September 18)

Parkinson’s disease is a frequent degenerative disorder, and for the moment the diagnosis is mainly clinical. When the first symptoms appear, loss of more than 70% of the dopaminergic cells already ... [more ▼]

Parkinson’s disease is a frequent degenerative disorder, and for the moment the diagnosis is mainly clinical. When the first symptoms appear, loss of more than 70% of the dopaminergic cells already occurred. Knowing that, it is of high interest to have one (or more) reliable biomarker(s) at our disposal to diagnose Parkinson before the first symptoms appear. Alpha-synuclein (aSyn) is a protein physiologically expressed at high level by neuronal cells, under a monomeric form. This protein would play a critical role in the development of the disease because under certain conditions, aSyn is capable of self-assembly to form fibrils like those found in Lewy bodies. Other intermediate soluble forms like dimers and oligomers are also formed. As these forms seems to be the toxic species, they are the center of many attentions. The quantification of each form would be a great help, but for the moment only the total forms (of monomeric or oligomeric) can be quantified. In this study, aSyn oligomers were generated after optimization of incubation conditions (pH, temperature, agitation, …). Then, different approaches were investigated to detect and follow the different species formed during the aggregation. We analyzed the oligomers by capillary gel electrophoresis (CGE) and SDS-PAGE. We found that capillary gel electrophoresis is a promising automated technique to analyze aSyn oligomers, due to the fact that it separates the aggregates according to their size, like the SDS-PAGE, but with more advantages. To gain sensitivity and selectivity by CGE, we used a laser-induced fluorescence detector. As aSyn do not have a native fluorescence, we derivatized it. After careful screening and optimization of various derivatization reagents, we could quantify with high sensitivity aSyn oligomers by CGE-LIF. We realized different calibration curves, and we had promising results that will allow us to quantify the different aSyn oligomeric forms in biological fluids. [less ▲]

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See detailA SPRAY DRYING METHOD FOR THE PREPARATION OF Na2FePO4F/CB AND Na2FePO4F/CNT COMPOSITES CATHODE FOR LITHIUM-ION BATTERIES
Mahmoud, Abdelfattah ULiege; Berardo, Loris ULiege; Brisbois, Magali et al

Poster (2017, September 18)

Fluorophosphates are considered among the most interesting series of cathode material for Li/Na-ion batteries1. Na2FePO4F (space group Pbcn), with its layered structure and two-dimensional pathways for ... [more ▼]

Fluorophosphates are considered among the most interesting series of cathode material for Li/Na-ion batteries1. Na2FePO4F (space group Pbcn), with its layered structure and two-dimensional pathways for facile Na+/Li+ transport, exhibits minimal structural changes upon reduction/oxidation. The average working voltage is 3.3 V vs. Li/Li+. Intercalation/deintercalation results in a volume change of only 3.7%. However, one of the key drawbacks of Na2FePO4F electrodes is their low intrinsic electronic conductivity. In order to study the effect of the carbon black and carbon nanotubes on the electrochemical performance of Na2FePO4F cathode material for lithium-ion batteries, Na2FePO4F, Na2FePO4F/CB and Na2FePO4F/CNT were prepared by a spray-drying method with different ratios of CB and CNT (10 and 20%). The crystal and local structure were analyzed by XRD and Mössbauer spectroscopy. The electrochemical properties were studied by galvanostatic cycling in lithium cells. The electrochemical performance is markedly better in the case of Na2FePO4F/CNT (20 wt%), with specific capacities of about 100 mAh/g (Na2FePO4F/CNT) at C/4 rate2 vs. 50 mAh/g for Na2FePO4F/CB. The characterization of Na2FePO4F/CB particles by electron microscopy revealed a carbon-poor surface and a good carbon dispersion for Na2FePO4F/CNT particles attributed to better diffusion of carbon nanotubes in the droplets during drying. References : 1-N. Eshraghi, S. Caes, A. Mahmoud, R. Cloots, B. Vertruyen, F. Boschini, Electrochim. Acta, 228 (2017) 319–324. 2-M. Brisbois, S. Caes, M-T. Sougrati, B. Vertruyen, A. Schrijnemakers, R. Cloots, N. Eshraghi, R-P. Hermann, A. Mahmoud, F. Boschini, Solar Energy Materials & Solar Cells 148 (2015) 67-72. [less ▲]

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See detailSharing good practices in physical education to promote physical activity, wellness and health
Bonni, Jérémy ULiege; Cloes, Marc ULiege

Poster (2017, September 15)

The Wallonia-Brussels Federation is about to overhaul its education system, which will directly affect teacher training. Under the “Pact for Excellence in Education”, a group of experts – the Consortium ... [more ▼]

The Wallonia-Brussels Federation is about to overhaul its education system, which will directly affect teacher training. Under the “Pact for Excellence in Education”, a group of experts – the Consortium “Physical Activities, Well-Being and Health” – drawn from the 12 French-speaking institutions training PE teachers (universities and colleges) has been mandated by the Ministry of Education to design devices and tools aiming at implementing the new common core curriculum that is under construction. Good practices have been identified then described in information sheets common to the different consortiums of the PEE and listed in a table that cross-references the 6 dimensions of physical activity at school (Snyers et al., 2014) and the 20 objects of teaching-learning in health education (Turcotte et al., 2011). These good practices are meant to be shared later within a community of practice. This paper provides a description of one of these information sheets in order to illustrate the methodology applied in this project. [less ▲]

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See detailMusic in the Operating Room
XHAUFLAIRE, Gaël ULiege; Albert, Louisa; Gengoux, Monique

Poster (2017, September 15)

Listening to music during surgery has a subjective benefit to patients under local anesthesia. Its setting up during quiet routine oculoplastic surgery is easy and inexpensive. When subject to some ... [more ▼]

Listening to music during surgery has a subjective benefit to patients under local anesthesia. Its setting up during quiet routine oculoplastic surgery is easy and inexpensive. When subject to some elementary precautions, there is no increased risk. [less ▲]

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See detailChanges in a metacognitive heuristic in amnesia
Thonnard, Adeline ULiege; Geurten, Marie ULiege; Willems, Sylvie ULiege

Poster (2017, September 15)

Amnesic patients rely predominantly on familiarity for recognition. This familiarity is thought to be based on processing fluency. However whether amnesic patients use normally this metacognitive ... [more ▼]

Amnesic patients rely predominantly on familiarity for recognition. This familiarity is thought to be based on processing fluency. However whether amnesic patients use normally this metacognitive heuristic is not yet known. We used the Jacoby and Whitehouse (1989)’s illusion paradigm with 8 patients with severe memory impairments and 16 matched control. We found a fluency effect, with more “old” responses being given to matched-prime test words than to mismatched-prime test words. In addition, we replicated the effect of modality (Westerman et al., 2002) in the control group. The fluency induced the recognition illusion, but only when there was a match between study and test sensory modality. However, this modality effect seems limited to control participants. Amnesic patients seem unable to disregard fluency when there was a shift in the sensory modality of the study and test stages. Overall, these findings support the notion that amnesic patients did not modulate their use of the fluency heuristic in situations where fluency is judged non-diagnostic of prior exposure by healthy participants. On the whole, these findings suggest that processing fluency can constitute a cue for recognition decision in amnesia. However, this influence is not direct and is subject to metamemorial belief changes. [less ▲]

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See detailExecutive functions in normal aging: the mediating effect of processing speed and attentional system
Gilsoul, Jessica ULiege; Simon, Jessica ULiege; Collette, Fabienne ULiege

Poster (2017, September 14)

Objective The normal aging is associated with decrease in executive functioning efficiency. However, the source of these cognitive difficulties could reside in some factors that are not executive per se ... [more ▼]

Objective The normal aging is associated with decrease in executive functioning efficiency. However, the source of these cognitive difficulties could reside in some factors that are not executive per se. Therefore, this present study is aimed at determining whether speed of processing and attentional variables would mediate the effect of age on the executive functioning. Participants and Methods Young (n = 104) and old (n = 63) participants were enrolled and carried out a large battery made of 21 executive (inhibition, shifting, working memory updating, and double task coordination), attentional (phasic alertness, selective attention, and sustained attention), and processing speed tasks. Based on Baron & Kenny’s recommendations (1986), mediation models of the age effect on the executive functioning were carried out with processing speed and attentional system taken as mediators. Results Selective attention significantly mediated the effect of age on working memory updating [􀇻R2 = 7%, p < .001]. Moreover, processing speed significantly mediated the effect of age on shifting [􀇻R2 = 7%, p < .001], working memory updating [􀇻R2 = 5%, p < .001], and double task coordination [􀇻R2 = 14%, p < .001]. Conclusions The mediating effect of age on most of the executive functions by measures of processing speed is in agreement with the literature showing a major influence of the slowdown of the processing speed on cognition (Salthouse, 1992; 2000; 2005). The age effect on inhibition did not seem to be mediated neither by the attentional variables nor the speed variables, which is in accordance with Zacks & Hasher’s hypothesis (1988). According to these authors, inhibition would be the first cognitive function to decline with age. [less ▲]

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See detailToward discovery of novel key genes for pancreatic beta cell regeneration in zebrafish
Carril Pardo, Claudio Andrès ULiege; Bergemann, David ULiege; Massoz, Laura ULiege et al

Poster (2017, September 14)

Diabetes is becoming a leading health issue worldwide. It occurs under insulin resistance/deficiency and when insulin-producing pancreatic beta-cell mass is dramatically reduced. Besides therapeutic ... [more ▼]

Diabetes is becoming a leading health issue worldwide. It occurs under insulin resistance/deficiency and when insulin-producing pancreatic beta-cell mass is dramatically reduced. Besides therapeutic strategies to preserve beta-cell mass and function and improve insulin treatments, beta-cell replacement constitutes a promising alternative to replenish the pancreas with functional beta-cells. Several observations of pancreatic cell plasticity has led to the hope that triggering beta-cell regeneration within the pancreas could be harnessed in future therapies. Still, mammals show limited regenerative capabilities, making difficult the study of these mechanisms. In contrast, zebrafish is extensively used for regeneration studies notably of beta-cells. We recently showed that the adult zebrafish ducts display characteristics of embryonic pancreatic progenitors that can give rise to beta-cells in physiological and induced diabetic condition*, and we determined the transcriptomic profile of zebrafish pancreatic ducts during beta-cell regeneration. To identify new genes crucial for beta-cell regeneration we plan to investigate the role of about 10 selected candidate genes by analyzing invalidating mutations created by CRISPR/Cas9. Their effect on beta-cell regeneration will be studied in 7-20 old larvae which we have established as experimental platform for functional studies. This project should help decipher the molecular mechanism of beta-cell regeneration. [less ▲]

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See detailFrom Metabolomics Study of Age-Related Macular Degeneration (AMD) to the Development of New Pyruvate Dehydrogenase Kinase Inhibitors (PDK)
Arslan, Deniz ULiege; Schoumacher, Matthieu ULiege; Pirotte, Bernard ULiege et al

Poster (2017, September 14)

Age-related macular degeneration (AMD) is a leading cause of blindness in the elderly population of industrialized countries. This blindness results from the deterioration of the macula, a small part of ... [more ▼]

Age-related macular degeneration (AMD) is a leading cause of blindness in the elderly population of industrialized countries. This blindness results from the deterioration of the macula, a small part of the retina specialized for the high-acuity vision. Exudative AMD, called “wet”, is characterized by the formation of new blood vessels growing under the retina according to a process named choroidal neovascularization (CNV). Currently, the aetiology and pathogenesis of AMD remain unclear. Nevertheless, a recent metabolomics study performed on the serum of “wet” AMD patients and on a CNV murine model, that mimics the effect of “wet” AMD, have demonstrated that lactate level is clearly involved in the severity of the pathology as well as the relationship between lactate, CNV and AMD [1]. According to this result, we suggest a new therapeutic approach of AMD based on the normalization of blood lactate level. The modulation of the lactate plasma concentration by treatment of the animals with synthetic compounds and more specifically Pyruvate Dehydrogenase Kinase (PDK) inhibitors significantly decrease the CNV. PDK and its four isoforms (PDK1-4) regulate the activity of the pyruvate dehydrogenase complex (PDH), a mitochondrial enzyme that plays a major role in the metabolic pathway of glucose, by reversible phosphorylation. Starting from these results, development of new PDK inhibitors could open the way to innovative treatment opportunities in AMD disease. Different analogues of (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropanamide (fig.1) have been already synthetized and pharmacological evaluation is currently in progress. According to the results obtained, various pharmacomodulations will be investigated. [less ▲]

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