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See detail(1)H, (13)C and (15)N assignments of a camelid nanobody directed against human alpha-synuclein.
Vuchelen, Anneleen; O'Day, Elizabeth; De Genst, Erwin et al

in Biomolecular NMR Assignments (2009), 3(2), 231-3

Nanobodies are single chain antibodies that are uniquely produced in Camelidae, e.g. camels and llamas. They have the desirable features of small sizes (Mw < 14 kDa) and high affinities against antigens ... [more ▼]

Nanobodies are single chain antibodies that are uniquely produced in Camelidae, e.g. camels and llamas. They have the desirable features of small sizes (Mw < 14 kDa) and high affinities against antigens (Kd approximately nM), making them ideal as structural probes for biomedically relevant motifs both in vitro and in vivo. We have previously shown that nanobody binding to amyloidogenic human lysozyme variants can effectively inhibit their aggregation, the process that is at the origin of systemic amyloid disease. Here we report the NMR assignments of a new nanobody, termed NbSyn2, which recognises the C-terminus of the intrinsically disordered protein, human alpha-synuclein (aS), whose aberrant self-association is implicated in Parkinson's disease. [less ▲]

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See detail1, 2, 3 façons d’évaluer des travaux pratiques d’Histologie
Multon, Sylvie ULiege; Crahay, Vinciane ULiege; Florquin, Sandra et al

Conference (2015, January)

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See detail1,2,4-Benzothiadiazine derivatives, their preparation and use
Pirotte, Bernard ULiege; Lebrun, P.; De Tullio, Pascal ULiege et al

Patent (1997)

Detailed reference viewed: 249 (10 ULiège)
See detail1,2,4-Benzothiadiazine derivatives, their preparation and use
De Tullio, Pascal ULiege; Nielsen, F. E.; Hansen, J. B. et al

Patent (1999)

Detailed reference viewed: 197 (7 ULiège)
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See detail1,2,4-Triazole-3-thione Compounds as Inhibitors of Dizinc Metallo-β-lactamases
Sevaille, Laurent; Gavara, Laurent; Bebrone, Carine et al

in ChemMedChem (2017), 12

Metallo-β-lactamases (MBLs) cause resistance of Gram-negative bacteria to β-lactam antibiotics and are of serious concern, because they can inactivate the last-resort carbapenems and because MBL ... [more ▼]

Metallo-β-lactamases (MBLs) cause resistance of Gram-negative bacteria to β-lactam antibiotics and are of serious concern, because they can inactivate the last-resort carbapenems and because MBL inhibitors of clinical value are still lacking. We previously identified the original binding mode of 4-amino-2,4-dihydro-5-(2-methylphenyl)-3H-1,2,4-triazole-3-thione (compound IIIA) within the dizinc active site of the L1 MBL. Herein we present the crystallographic structure of a complex of L1 with the corresponding non-amino compound IIIB (1,2-dihydro-5-(2-methylphenyl)-3H-1,2,4-triazole-3-thione). Unexpectedly, the binding mode of IIIB was similar but reverse to that of IIIA. The 3 D structures suggested that the triazole-thione scaffold was suitable to bind to the catalytic site of dizinc metalloenzymes. On the basis of these results, we synthesized 54 analogues of IIIA or IIIB. Nineteen showed IC50 values in the micromolar range toward at least one of five representative MBLs (i.e., L1, VIM-4, VIM-2, NDM-1, and IMP-1). Five of these exhibited a significant inhibition of at least four enzymes, including NDM-1, VIM-2, and IMP-1. Active compounds mainly featured either halogen or bulky bicyclic aryl substituents. Finally, some compounds were also tested on several microbial dinuclear zinc-dependent hydrolases belonging to the MBL-fold superfamily (i.e., endonucleases and glyoxalase II) to explore their activity toward structurally similar but functionally distinct enzymes. Whereas the bacterial tRNases were not inhibited, the best IC50 values toward plasmodial glyoxalase II were in the 10 μm range. [less ▲]

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See detail1,6-AnhMurNAc derivatives for assay development of amidase AmiD.
Mercier, Frédéric ULiege; Zervosen, Astrid ULiege; Teller, Nathalie et al

in Bioorganic & Medicinal Chemistry (2010), 18(21), 7422-31

Various peptidoglycan fragments were synthesized from two anhydro-muramic acid derivatives protected with a Bn or a PMB group at the 4th position, in homogenate phase or on a solid support. In order to ... [more ▼]

Various peptidoglycan fragments were synthesized from two anhydro-muramic acid derivatives protected with a Bn or a PMB group at the 4th position, in homogenate phase or on a solid support. In order to facilitate HPLC detection, a chromophoric group was attached to the peptide chain. The periplasmic amidase sAmiD of Escherichia coli was used to cleave the amide bond between the lactyl group of the MurNAc and the alpha-amino group of L-Ala where the peptide chain was at least a dipeptide (L-Ala-gamma-D-Glu) amidated by benzylamine on the gamma-carboxyl group of D-Glu. In the presence of a tripeptide chain (L-Ala-gamma-D-Glu-L-Lys) or a tetrapeptide chain (L-Ala-gamma-D-Glu-m-A(2)pm-D-Ala) higher hydrolysis rates were observed. We have also demonstrated that the presence of TNB on the epsilon-amino group of L-Lys only has a small influence on the hydrolysis capacity of sAmiD. [less ▲]

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See detail[1-11C]Bromoethane: an alternative to the use of [1-11C]iodoethane.
Schmitz, F.; Del Fiore, G.; Plenevaux, Alain ULiege et al

in Journal of Labelled Compounds & Radiopharmaceuticals (1995), 37

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See detail1-84 PTH and KDIGO Guidelines
CAVALIER, Etienne ULiege

Conference (2012, April 18)

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See detail1-anilino-8-naphtalene sulfonate probes a gastric HK-ATPase potassium site whose access requires ionophores.
Festy, F.; Lins, Laurence ULiege; Gallet, X. et al

in Journal of Membrane Biology (1998), 165(2), 153-60

1-anilino-8-naphtalenesulfonate (ANS) is a hydrophobic dipole previously used to demonstrate that the proton for potassium exchange by the gastric HK-ATPase is electroneutral. In this paper, we ... [more ▼]

1-anilino-8-naphtalenesulfonate (ANS) is a hydrophobic dipole previously used to demonstrate that the proton for potassium exchange by the gastric HK-ATPase is electroneutral. In this paper, we demonstrate that ANS binds to gastric membranes and probes conformational changes of the HK-ATPase independently of any active H for K exchange. Conformational changes require the presence of potassium-valinomycin and are not triggered by sodium. Potassium effect is enhanced by ATP, in the presence and in the absence of magnesium and, by ADP, in the presence of magnesium. Labeling of the pig HK-ATPase K518 by fluorescein-5-isothiocyanate inhibits the enzyme activity and knocks out the ATP effect on ANS fluorescence. Scherring 28080 and the monoclonal antibody 95-111, two competitive inhibitors of K-activated ATPase dephosphorylation, do not modify K-effect on ANS fluorescence but inhibit ATP effects. This supports that ANS does not probe K-site between the H1-H2 loop. Treatment of gastric membranes with trypsin does not inhibit the ANS response to potassium but does inhibit the response to ATP. This suggests that the ATP site inducing the ANS response is cytoplasmic and the potassium site is intramembranous. Titration reveals that one mole of ANS interacts with one mole of ATPase. We suggest that ANS probes a hydrophobic potassium site of gastric ATPase and that addition of ATP and ADP-Mg embed that site. [less ▲]

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See detail1-D shallow water model for industrial practice: Application to the River Romanche
Goffin, Louis ULiege; Dewals, Benjamin ULiege; Erpicum, Sébastien ULiege et al

Scientific conference (2016, October 25)

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See detail1-Year Controlled Randomised Trial of Prevention of Early Postmenopausal Bone Loss by Intranasal Calcitonin
Reginster, Jean-Yves ULiege; Denis, D.; Albert, Adelin ULiege et al

in Lancet (1987), 2(8574), 1481-3

79 women who had been menopausal for less than 36 months and who had not received any form of treatment to prevent bone loss were randomly assigned to a 12-month regimen of calcium 500 mg/day or calcium ... [more ▼]

79 women who had been menopausal for less than 36 months and who had not received any form of treatment to prevent bone loss were randomly assigned to a 12-month regimen of calcium 500 mg/day or calcium 500 mg plus intranasal salmon calcitonin 50 IU/day for 5 days per week. After 12 months of treatment bone mineral density had decreased in the calcium-only group by a mean of 3.16 (SEM 0.6)% (p less than 0.01) but had increased in the calcium plus calcitonin group by 1.38 (0.8)% (NS). The difference in response between the two treatment groups was also highly significant (p less than 0.01), as was the difference between values for hydroxyprolinuria/creatininuria (p less than 0.01). Endogenous calcitonin levels rose significantly in the calcium group but remained unchanged in calcitonin-treated patients. Treatment by calcitonin and calcium was not followed by increased secretion of parathyroid hormone. The findings suggest that intranasal calcitonin can counteract early postmenopausal bone loss. [less ▲]

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See detail1. Actor Dynamics and Sustainable Development: Emerging Roles of Researchers
Bryant, Christopher; Bousbaine, Antonia ULiege

in revue canadienne de géographie tropicale (2014), Vol 1(N° 2 1-5), 1-5

The challenges facing sustainable development are essentially human-based; human society has mainly created or exacerbated many human problems such as poverty and food security as well as natural ... [more ▼]

The challenges facing sustainable development are essentially human-based; human society has mainly created or exacerbated many human problems such as poverty and food security as well as natural environment problems, such as climate change and climate extremes. In addition, the eventual solutions must be elaborated and put into place by various actors. Thus it is essential to have a thorough understanding of actor dynamics … the interests of different actors, interactions between them and the initiatives that they engage in. A framework is presented to help any observer and researcher in any cultural or territorial context to understand the challenges facing the involvement of actors and human society in general in any search for sustainable development, and this is so for the countries of the North and the South. We then explore how a better understanding of actor dynamics support those researchers who wish to make a substantial contribution to resolving these challenges and helping populations move closer towards a sustainable future for their communities and environments. It is argued that one of the most pertinent ways for researchers to do this is through the various research approaches termed action research; it is argued that with patience and playing out the roles of counselor, facilitator, and provider of strategic information, action research, particularly Participatory Action Research (PAR) can achieve significant progress towards a sustainable future. [less ▲]

Detailed reference viewed: 55 (1 ULiège)